Instrumental Methods in Biomedicine II

Relations:

In order to register for the course F7PMLIMB2, the student must have successfully completed or received credit for and not exhausted all examination dates for the course F7PMLIMB1. The course F7PMLIMB2 can be graded only after the course F7PMLIMB1 has been successfully completed.

In order to register for the course F7PMLILP2, the student must have successfully completed the course F7PMLIMB2.

Course guarantor:

Alena Zavadilová

Lecturer:

Martin Vlk, Alena Zavadilová

Tutor:

Alena Zavadilová

Supervisor:

Department of Health Care Disciplines and Population Protection

Synopsis:

Requirements:

The course is completed with an examination, which consists of a written and an oral part taking place on the same day. A prerequisite for admission to the oral examination is the successful completion of the written part. The oral part is graded. All questions are drawn at random, and preparation time is provided. In the case of a grade F, both parts of the examination must be retaken.

During the first half of the semester, only lectures take place in the INSN 1 and PINS time slots, while in the second half of the summer semester, only practical classes are held in both time slots.

Syllabus of lectures:

Overview of basic types of radiation sources and detectors used in basic techniques of analytical methods, principles of these methods, introduction to selected advanced instrumental methods of research and analysis, utilization and application.

Outline:

1.Overview of basic radiation sources and principles for X-ray, UV-VIS, IR

2.Overview of basic detectors and principles of detection

3.Basics of optics and spectroscopy

4.Basics of vacuum technology (sources, measurements)

5.Selected analytical methods

UV-VIS absorption spectrometry

Atomic absorption spectrometry

Infrared absorption spectroscopy (basic FTIR techniques)

Absorption methods on beams of synchrotron radiation

Raman spectroscopy

Methods based on emission of radiation (Luminescent spectrometry (TRLFS, LIBS, etc.), X-ray photoelectron spectroscopy)

Mössbauer spectroscopy

Nuclear magnetic and paramagnetic resonance

Chromatographic analytical methods (High performance liquid chromatography, Gas chromatography)

Mass spectrometry (MS)

Thermal analysis methods (eg differential thermal analysis, thermogravimetry, DSC, TD, EGA, ETA)

Modern microscopy ( electron microscopy, fluorescence microscopy (SIM, STED, STORM, etc.)

Syllabus of tutorials:

Study Objective:

Study materials:

Note:

Time-table for winter semester 2025/2026:

Time-table is not available yet

Time-table for summer semester 2025/2026:

	06:00–08:0008:00–10:0010:00–12:0012:00–14:0014:00–16:0016:00–18:0018:00–20:0020:00–22:0022:00–24:00
Mon
Tue
Wed	roomFJFI Zavadilová A. Vlk M. 09:00–11:50 (lecture parallel1) Trója, těžké laboratoře, V Holešovičkách 2
Thu
Fri

The course is a part of the following study plans:

• Biomedical Laboratory Methods (compulsory course)