Microbiology and Imunology

Relations:

In order to register for the course F7PBOMI, the student must have successfully completed the course F7PBOBLG in a previous semester.

Course guarantor:

Taťána Jarošíková

Lecturer:

Taťána Jarošíková, Kristýna Marková, Hana Vrbová

Tutor:

Taťána Jarošíková, Kristýna Marková, Hana Vrbová

Supervisor:

Department of Natural Sciences

Synopsis:

Immunology: Cells and organs of the immune system. Antigens. Development of the immune response. The main histocompatibility complex. T lymphocytes and cellular immunity. Cellular cytotoxicity. Cytokines. B lymphocytes and antibody production. Immunoglobulins. Defense functions of the immune system. Anti-infective immunity. Innate immunity. Allergic diseases. Nutrition and immunity, the effect of human microbiome on the immune system. Immunopathology. Immunodeficiency. Autoimmune diseases.

Microbiology: Microorganisms, division. Non-cellular forms of infections - viruses. Procaryotes. Bacterial cell structure and function. Phylogenetic system of bacteria and archaea. Eucaryotes. Cell structure and function of eukaryotic microorganisms - fungi, protozoa. Metabolism and growth of microorganisms, life cycle of prokaryotic cells, growth curve. Influence of environmental factors on the growth of microorganisms - temperature, pH. Antimicrobials - antivirals, antibiotics and chemotherapeutics, mechanism of action, disinfection, sterilization. The microbiome of the human body. Human microbial diseases. Infectious diseases of the eye caused by microorganisms - viral, bacterial, fungal and caused by protozoa.

Requirements:

Requirements for the graded credit:

1. Participation in exercises; in case of absence, an excuse must be submitted and the exercise must be made up by agreement with the instructor.

2. Successful completion of the ongoing and final test (includes 30 questions); to be awarded the credit, the student must have 60% correct answers in both tests.

Syllabus of lectures:

1. Immunology: Cells and organs of the immune system.

2. Antigens. MHC and HLA system

3. Non-specific immunity - cellular and humoral components. (extent of 1 hour/semester).

4. Inflammation. Cytokines

5. Specific immunity.

6. Immunization. Anti-infective immunity

7. Allergic disease. Nutrition and immunity, the influence of the human microbiome on the immune system.

8. Transplantation. Immunopathology. Immunodeficiency.

9. Microbiology: Microorganisms, division. Non-cellular forms of infection - viruses. Prokaryote.

10. Phylogenetic system of bacteria and archaea. Nutrition and growth of microorganisms.

11. Influence of factors on the growth of microorganisms - temperature, pH. Antimicrobial, antibacterial agents - antibiotics and their mechanism of action, disinfection, sterilization.

12. Genetics and molecular biology of microorganisms. Microbial diseases

13. Human microbial diseases

14. Microflora of the human body. Eye infection.

Syllabus of tutorials:

1. Work in a microbiology laboratory.

2. Methods of sterilization and disinfection. Preparation of media.

3. Cultivation of bacteria. Isolation of bacteria.

4. Growth curve. Cell counting.

5. Evaluation of growth curve. Bacterial staining.

6. Immunological tests.

7. Graded credit - test and awarding

Study Objective:

Knowledge, skills, abilities and competences: overview and knowledge in the areas of general microbiology and immunology needed for further study. Ability to use clear microbiological and immunological knowledge in optics and optometry - infectious diseases of the eye.

Study materials:

[1] NORTHROP, Robert B. a Anne N. CONNOR. Introduction to molecular biology, genomics, and proteomics for biomedical engineers. Boca Raton: CRC Press/Taylor & Francis, 2009.

[2] ALBERTS, Bruce et al. Molecular biology of the cell: reference edition. 5th ed. New York: Garland Science, 2008.

[3] LODISH, Harvey F. et al. Molecular cell biology. 6th ed. New York: W. H. Freeman, 2008.

Note:

Time-table for winter semester 2025/2026:

Time-table is not available yet

Time-table for summer semester 2025/2026:

	06:00–08:0008:00–10:0010:00–12:0012:00–14:0014:00–16:0016:00–18:0018:00–20:0020:00–22:0022:00–24:00
Mon
Tue	roomKL:B-223 Vrbová H. 08:00–09:50 (lecture parallel1) Kladno FBMI roomKL:B-223 Marková K. 10:00–11:50 (lecture parallel1) Kladno FBMI roomKL:B-223 Marková K. 12:00–13:50 (lecture parallel1) Kladno FBMI roomKL:B-223 Marková K. 14:00–15:50 (lecture parallel1) Kladno FBMI roomKL:B-220 Marková K. Jarošíková T. 14:00–15:50 (lecture parallel1) Kladno FBMI
Wed
Thu	roomKL:B-223 Vrbová H. 08:00–09:50 (lecture parallel1) Kladno FBMI roomKL:B-223 Vrbová H. 14:00–15:50 (lecture parallel1) Kladno FBMI
Fri

The course is a part of the following study plans:

• Optics and Optometry (compulsory course)