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CZECH TECHNICAL UNIVERSITY IN PRAGUE
STUDY PLANS
2022/2023
UPOZORNĚNÍ: Jsou dostupné studijní plány pro následující akademický rok.

Cellular and Molecular Diagnostics

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Code Completion Credits Range Language
F7PMIBMD-N Z,ZK 3 2P+2L Czech
Garant předmětu:
Eva Neuhöferová
Lecturer:
Eva Neuhöferová
Tutor:
Eva Neuhöferová
Supervisor:
Department of Natural Sciences
Synopsis:

The students will obtain information regarding basic diagnostic methods used in cell biology with particular focus on laboratory training.

Requirements:

Basic (university) knowledge of cellular and molecular biology

Syllabus of lectures:

Theory:

1.Cell, multicellular organism, microenvironment

2.Intracellular signal transduction

3.Laboratory models in vitro and in vivo

4.Methods for assesments of cell viability, proliferation, and death

5.Methods for analyses of surface and cytoplasmic markers

6.Tumor antigens and cytokine production

Syllabus of tutorials:

Laboratory training:

1.Detection of cell prosperity and plasma membrane permeability

Tasks: Cell counting, WST-1 assay, LDH assay

2.2D a 3D tumor model development

Task: Tissue cultures

3.Specific cells detection within tumor microenvironment using fluorescent microscopy or flow cytometry

Task: Surface and cytoplasmic antigens staining

Study Objective:

We will introduce students to experimental techniques used in cell biology laboratories and obtaining basic laboratory practice.

Study materials:

Basic literature:

Václav Hořejší, Jiřina Bartůňková: Základy imunologie. Triton, 4. vyd., 2009

Oldřich Nečas: Obecná biologie pro lékařské fakulty. 1. vyd. Jinočany: H&H, 2000.

Recommended literature:

Donald Voet, Judith G. Voet: Biochemie. Victoria Publishing 2005.

Note:
Time-table for winter semester 2022/2023:
Time-table is not available yet
Time-table for summer semester 2022/2023:
06:00–08:0008:00–10:0010:00–12:0012:00–14:0014:00–16:0016:00–18:0018:00–20:0020:00–22:0022:00–24:00
Mon
roomMBUCAS
Neuhöferová E.
14:00–15:50
(lecture parallel1)
Krč
Mikrobiologický ústav AV ČR
roomMBUCAS
Neuhöferová E.
16:00–17:50
(lecture parallel1
parallel nr.1)

Krč
Mikrobiologický ústav AV ČR
Tue
Wed
Thu
Fri
The course is a part of the following study plans:
Data valid to 2023-06-02
Aktualizace výše uvedených informací naleznete na adrese https://bilakniha.cvut.cz/en/predmet6401706.html