Cellular and Molecular Diagnostics
| Code | Completion | Credits | Range | Language |
|---|---|---|---|---|
| F7PMIBMD-N | Z,ZK | 3 | 2P+2L | Czech |
- Garant předmětu:
- Eva Neuhöferová
- Lecturer:
- Eva Neuhöferová
- Tutor:
- Eva Neuhöferová
- Supervisor:
- Department of Natural Sciences
- Synopsis:
-
The students will obtain information regarding basic diagnostic methods used in cell biology with particular focus on laboratory training.
- Requirements:
-
Basic (university) knowledge of cellular and molecular biology
- Syllabus of lectures:
-
Theory:
1.Cell, multicellular organism, microenvironment
2.Intracellular signal transduction
3.Laboratory models in vitro and in vivo
4.Methods for assesments of cell viability, proliferation, and death
5.Methods for analyses of surface and cytoplasmic markers
6.Tumor antigens and cytokine production
- Syllabus of tutorials:
-
Laboratory training:
1.Detection of cell prosperity and plasma membrane permeability
Tasks: Cell counting, WST-1 assay, LDH assay
2.2D a 3D tumor model development
Task: Tissue cultures
3.Specific cells detection within tumor microenvironment using fluorescent microscopy or flow cytometry
Task: Surface and cytoplasmic antigens staining
- Study Objective:
-
We will introduce students to experimental techniques used in cell biology laboratories and obtaining basic laboratory practice.
- Study materials:
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Basic literature:
Václav Hořejší, Jiřina Bartůňková: Základy imunologie. Triton, 4. vyd., 2009
Oldřich Nečas: Obecná biologie pro lékařské fakulty. 1. vyd. Jinočany: H&H, 2000.
Recommended literature:
Donald Voet, Judith G. Voet: Biochemie. Victoria Publishing 2005.
- Note:
- Time-table for winter semester 2022/2023:
- Time-table is not available yet
- Time-table for summer semester 2022/2023:
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06:00–08:0008:00–10:0010:00–12:0012:00–14:0014:00–16:0016:00–18:0018:00–20:0020:00–22:0022:00–24:00
Mon Tue Wed Thu Fri - The course is a part of the following study plans:
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- Nanotechnology (compulsory course)