Fundamentals of Immunology and Immunochemistry

Relations:

In order to register for the course F7PBLKIM, the student must have successfully completed the course F7PBLZII.

In order to register for the course F7PBLLPR, the student must have successfully completed the course F7PBLZII.

Course guarantor:

Taťána Jarošíková

Lecturer:

Taťána Jarošíková

Tutor:

Romana Široká

Supervisor:

Department of Natural Sciences

Synopsis:

tissues, cells, and molecules, as well as the mechanisms of natural and acquired immunity. They will also learn about the theoretical basis of immune function disorders, immunodeficiency states, autoimmunity, and hypersensitivity states.

During practical exercises, they will learn the theoretical and practical principles of selected immunological examination methods, their implementation, and evaluation.

Requirements:

Conditions for obtaining credit:

1. 2 tests minimum 60% success rate (dates 1st after week 7, 2nd after week 13).

2. 100% attendance at practical exercises.

3. All reports from practical tasks submitted.

Conditions for the exam:

1. Successful completion of a written test, 25 questions with 1 correct answer to choose from, or filling in terms, or matching questions and answers. To proceed to the oral exam, a minimum test success rate of 60% is required.

2. Oral examination on the material covered.

Syllabus of lectures:

Immunology basic concepts and functions.

Organs and tissues of the immune system.

Cells and molecules involved in the immune response.

Non-specific immunity cellular components (phagocytes, phagocytosis).

Non-specific immunity humoral components (complement system).

Inflammation.

B lymphocytes. Immunoglobulins and B cell receptors.

T cell biology, antigen presentation, T cell function.

Cytokines and chemokines.

Anti-infective immunity.

Immunization.

Immunopathological states of hypersensitivity allergies.

Transplantation.

Immunodeficiency.

Syllabus of tutorials:

Principles of work in an immunology laboratory. Work safety.

Antibodies as immunological reagents. Monoclonal antibodies, use in laboratory diagnostics. (S)

Antigen-antibody reactions in vitro. Primary and secondary interactions. Overview of serological reactions. Specificity and sensitivity. Serum titration. (L)

Direct and indirect agglutination reactions. (L)

Precipitation reactions. (L)

Turbidimetric methods. Blotting methods. Western blot. (L)

Enzyme immunoassays (ELISA). (L)

RIA. IgE detection. (L)

Complement system testing. Detection of circulating and deposited immune complexes. RVK. (L)

Flow cytometry. Lymphocyte function tests. (S)

Immunofluorescence. Use in antigen identification and antibody detection. (S)

Theoretical basis of allergic reactions. (S)

In vitro tests for the detection of allergic sensitization. (L)

Immunoelectrophoretic methods. Immunofixation. (S)

Credit test.

Study Objective:

Study materials:

Povinná literatura:

HOŘEJŠÍ, Václav, Jiřina BARTŮŇKOVÁ, Tomáš BRDIČKA a Radek ŠPÍŠEK. Základy imunologie. 6., aktualizované vydání. Praha: Triton, 2017. ISBN 978-80-7553-250-3.

JÍLEK, Petr. Imunologie: stručně, jasně, přehledně. 4., přeprac. a dopl. vyd. Praha: Grada, 2019. ISBN 978-80-271-0595-3.

BARTŮŇKOVÁ, Jiřina a Milan PAULÍK. Vyšetřovací metody v imunologii 2. přeprac. a dopl. vyd. Grada, 2011. ISBN 978-80-247-3533-7.

Doporučená literatura:

FERENČÍK, Miroslav, Jozef ROVENSKÝ, Vladimír MAŤHA a Jaroslav UTĚŠENÝ. Ilustrovaný slovník imunologie a alergologie. 2. přeprac. a dopl. vyd. Praha: Galén, 2011. ISBN 978-80-7262-762-2.

DETTMER Philipp, Podnázev: A Journey into the Mysterious System That Keeps You Alive. Nakladatelství Hodder and Stoughton, 368 s. 2021.

ISBN: 978-152-936-068-4

Note:

Time-table for winter semester 2025/2026:

	06:00–08:0008:00–10:0010:00–12:0012:00–14:0014:00–16:0016:00–18:0018:00–20:0020:00–22:0022:00–24:00
Mon	roomKL:B-420 Jarošíková T. 12:00–13:50 (lecture parallel1) Kladno FBMI roomKL:A-s111 Široká R. 18:00–19:50 (lecture parallel1) Kladno FBMI roomKL:B-224 Široká R. 14:00–15:50 (lecture parallel1) Kladno FBMI roomKL:A-s111 Široká R. 16:00–17:50 (lecture parallel1) Kladno FBMI
Tue
Wed
Thu
Fri

Time-table for summer semester 2025/2026:

Time-table is not available yet

The course is a part of the following study plans:

• Medical Laboratory Diagnostics (compulsory course)