Analytical Chemistry 2
- Garant předmětu:
- Department of Nuclear Chemistry
Analytical chemistry II is the continuation of lecture Analytical chemistry I. This course is oriented to the instrumental methods of analysis and processing of analytical results.
Knowledge of general chemistry on the level of a completed basic university course. Completed course „Analytical Chemistry I“.
- Syllabus of lectures:
1. Absolute and comparative analytical methods. Calibration methods
(calibration curve method, standard addition method).
2. Basic statistical evaluation of data (accuracy and precision, mean, standard
deviation, confidence interval, valid numbers).
3. Electroanalytical methods based on measurement of potential. Equilibrium
potentiometry (electrochemical cell, electrodes). Direct potentiometry
(measurement of pH, determination of other ions using ISE, selectivity
coefficient, gas sensors based on ISE). Indirect potentiometry, potentiometric
4. Electroanalytical methods based on measurement of current I. Voltammetry and
polarography (polarization curve, scheme of electrochemical cell, electrodes,
dc, ac and pulse voltammetry. Voltammetric stripping analysys. Amperometry
5. Electroanalytical methods based on measurement of current II.
Electrogravimetry and coulometry (potentiostatic and galvanostatic methods,
coulometric titrations, colometric analyzers). Conductometry (conductometric
analyzers and detectors).
6. Optical methods (the nature of absorption and emission of radiation by atoms
and molecules). Atomic and molecular absorption and emission/fluorescence
spectra, their utilization in analytical chemistry. Molecular absorption
spectrometry in uv/vis range of radiation. Atomic absorption spectrometry.
7. Automatic chemical analysers (FIA, CFA). Nonspectral optical methods
(refractometry, polarimetry, nephelometry).
8. Chromatographic methods (fundamentals, classification of chromatographic
methods). Planar chromatography. Column chromatography (general aspects,
parameters obtained from chromatogram, separation efficiency, resolution,
optimization of separation process). Application of chromatographic methods in
quantitative and qualitative analysis (hyphenated methods, GC-MS, LC-MS).
9. Gas chromatography (columns, detectors). Liquid chromatography (selection of
mobile phase, classification of LC based on difference in stationary phase -
partition, affinity, size-exclusion, ion-exchange chromatography).
10. Electromigration methods. Separation in electric field (zone and capillary
electrophoresis). Example of separation - aminoacids and peptides.
- Syllabus of tutorials:
1. Acid-base equilibria and pH calculations. Acid-base titrations and stochiometry.
2. Redox equilibria, titrations and stochiometry.
3. Basic principles and calculations in separation methods.
4. Statistics of experimental analytical results.
- Study Objective:
The course of analytical chemistry provides the students with the knowledge of the basic methods of analytical chemistry and schemes of analytical procedures.
The students will acquire competence to select independenty optimum analytical approach to the given problem.
- Study materials:
1. D.A.Skoog, D.M.West and F.J.Holler, Fundamentals of Analytical Chemistry, 6.
Edition, Saunders College Publishing, 1992;
1. Kellner R., Mermet J.M., Otto M., Widmer H.M. (Editors): Analytical
Chemistry, Willey-VCH, Weinheim 1998 (ISBN 3-527-28610-1).
- Further information:
- No time-table has been prepared for this course
- The course is a part of the following study plans:
- BS Jaderná chemie (compulsory course of the specialization)