Laboratory Practice in Analytical Chemistry

Course guarantor:

Lecturer:

Tutor:

Supervisor:

Department of Nuclear Chemistry

Synopsis:

First part of laboratory exercises is oriented to qualitative analysis of cations and anions using wet chemistry procedures. Quantitative determination of analyte based upon various titration procedures follows. In the last part of exercises students become acquainted with basic instrumental methods of chemical analysis.

Requirements:

Knowledge of analytical chemistry on the level of a completed basic university course.

Syllabus of lectures:

Syllabus of tutorials:

1. Qualitative analysis of inorganic cations and anions using wet chemistry: identification of two cations in mixture, identification of two anions in mixture.

2. Qualitative analysis of inorganic solid substance using wet chemistry: identification of cation and anion in a well-soluble and a bad-soluble solid sample.

3. Acid-base titrations: identification of analyte (sodium or potassium hydroxide or hydrochloric, sulfuric or nitric acid) in the sample, preparation of standard solution of hydrochloric acid or sodium hydroxide, acidimetry or alkalimetry of analyte in the sample.

4. Precipitation titrations: argentometry of sodium chloride in a liquid sample with Fajans' indication, titration of potassium iodide and chloride in a solid sample using potentiometric indication, titration curve construction and evaluation.

5. Chelatometry: identification of analytes in the sample, determination of magnesium and calcium ions in water as sample, titration of magnesium and zinc ions or lead and bismuth ions in mixture.

6. Iodometry: titration of ascorbic acid in Celaskon pellet, iodometry of acetone in liquid sample based on indirect titration.

7. Permanganometry: preparation of standard solution of potassium permanganate using oxalic acid as the standard, titration of iron in a solid sample using potentiometric indication, titration curve construction and evaluation.

8. Coulometry: determination of hydroquinone in a liquid sample with coulometric titration, calculation of analyte concentration from the electrical charge.

9. Potentiometry with ion selective electrode: determination of nitrate or fluoride ions using ion selective electrode, calibration curve construction.

10. Gas chromatography: separation of oxygen and nitrogen from air sample, evaluation of chromatogram and calculation of fundamental chromatographic quantities.

11. Spectrophotometry: spectrophotometric determination of acetylsalicylic acid in Acylpyrin pellet, calibration curve construction.

12. Solid phase extraction: preconcentration of iron ions from spring water using solid phase extraction, spectrophotometric determination of preconcentrated analyte, calibration curve construction.

13. Test in theory and practical exam with real sample.

Study Objective:

The laboratory practice in analytical chemistry provides the students with the knowledge, skills, proficiency and thoroughness in elementary and instrumental analytical techniques.

The students will acquire competence to perform practical laboratory analytical work, including data processing, and evaluation and presentation of the results.

Study materials:

Key literature:

1. http://www.natur.cuni.cz/analchem/praktika.html

Recommended literature:

1. G.D. Christian: Analytical Chemistry (5th edition), John Wiley & Sons, New York, 1994 (ISBN 0-471-30582-0).

2. R. Kellner, J.M. Mermet, M. Otto, H.M. Widmer (editors): Analytical Chemistry, Willey-VCH, Weinheim, 1998 (ISBN 3-527-28610-1).

Note:

Further information:

No time-table has been prepared for this course

The course is a part of the following study plans: