Immunopathology
Code | Completion | Credits | Range |
---|---|---|---|
15IMPL | ZK | 2 | 2+0 |
- Garant předmětu:
- Lecturer:
- Tutor:
- Supervisor:
- Department of Nuclear Chemistry
- Synopsis:
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The course is focused on fundamentals of regulation of innate immunity and specific immunity functions. Mechanisms of mucosal immune response, auto- immunity response, anti-infective immunity, hypersensitivity and molecular mechanisms of primary immunodeficiencies are thoroughly studied. Fundamental knowledge of cell-level immunology is expected as well as classification and
details of human diseases characterized by malfunction of immunity functions. Laboratory exercises demonstrate immunology methods used in clinical praxis.
- Requirements:
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The basis of knowledge of cell and molecular immunology
- Syllabus of lectures:
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1. Antibody immune response and regulation. T-independent, T-dependent antigens. Immunology basis of vaccination.
2. Immune function of T cells - Th1, Th2, Th17, regulatory T cells. Cytotoxic reactions, NK, NKT cells.
3. Mucosal and skin immune reaction. Inflammation and immune reaction.
4. Break of the immune tolerance, autoimmunity-mechanisms and risk factors.
5. Antiinfection immunity. Prions and the immune system. Primary immunodeficiency - molecular basis.
6. Immunopathology reactions of hypersensitivity, Coombs and Gell Type I - IV.
- Syllabus of tutorials:
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Practicum I:
Immune proteins analysis - immunoglobulins, specific antibodies (against infectious and other antigens), acute phase proteins, paraproteins
Methods for detection of autoantibodies - indirect immunofluorescence, ELISA, Western Blott (WB)
Complement tests, tests of the complement function (including complement inhibitors)
Theoretical principles of: immunodifusion, precipitation, agglutination, immunoelectrophoresis / immunofixation, radio/enzymoimmunoanalysis, turbidimetry and nephelometry.
Practical demonstration: typical patterns of autoantibodies detection by indirect immunofluorescence and westernblott
Based on case studies
Practicum II:
Methods for analysis of cell mediated immunity
Flow cytometry - principle of the method. Phenotypization of the cells - application in different areas. Analysis of the activation state of leucocytes, thrombocytes and other cells.
Functional tests of lymphocytes - cytotoxicity, proliferation, cytokine secretion
Functional tests of phagocyting cells - ingestion, metabolic burst of Neu
Practical demonstration: analysis of membrane markers of peripheral blood lymphocytes
Based on case studies
- Study Objective:
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The goal of the Course is to get knowledge for understanding of pathofysiological characteristics of abnormal immune reactions, to get the competence to explain mechanisms of immune mediated diseases.
- Study materials:
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Key literature:
Roitt I, Delves PJ: Essential Immunology (Blackwell Scientific Publications
2001, 10th ed)
Recommended literature:
Kuby J: Immunology, New York : W.H. Freeman, 1992
Immunology-10th ed. - Blackwell Scientific Publications, 2001.
Immunology-10th ed. - Blackwell Scientific Publications, 2001.
- Note:
- Further information:
- No time-table has been prepared for this course
- The course is a part of the following study plans: