Immunopathology

Garant předmětu:

Lecturer:

Tutor:

Supervisor:

Department of Nuclear Chemistry

Synopsis:

The course is focused on fundamentals of regulation of innate immunity and specific immunity functions. Mechanisms of mucosal immune response, auto- immunity response, anti-infective immunity, hypersensitivity and molecular mechanisms of primary immunodeficiencies are thoroughly studied. Fundamental knowledge of cell-level immunology is expected as well as classification and

details of human diseases characterized by malfunction of immunity functions. Laboratory exercises demonstrate immunology methods used in clinical praxis.

Requirements:

The basis of knowledge of cell and molecular immunology

Syllabus of lectures:

1. Antibody immune response and regulation. T-independent, T-dependent antigens. Immunology basis of vaccination.

2. Immune function of T cells - Th1, Th2, Th17, regulatory T cells. Cytotoxic reactions, NK, NKT cells.

3. Mucosal and skin immune reaction. Inflammation and immune reaction.

4. Break of the immune tolerance, autoimmunity-mechanisms and risk factors.

5. Antiinfection immunity. Prions and the immune system. Primary immunodeficiency - molecular basis.

6. Immunopathology reactions of hypersensitivity, Coombs and Gell Type I - IV.

Syllabus of tutorials:

Practicum I:

Immune proteins analysis - immunoglobulins, specific antibodies (against infectious and other antigens), acute phase proteins, paraproteins

Methods for detection of autoantibodies - indirect immunofluorescence, ELISA, Western Blott (WB)

Complement tests, tests of the complement function (including complement inhibitors)

Theoretical principles of: immunodifusion, precipitation, agglutination, immunoelectrophoresis / immunofixation, radio/enzymoimmunoanalysis, turbidimetry and nephelometry.

Practical demonstration: typical patterns of autoantibodies detection by indirect immunofluorescence and westernblott

Based on case studies

Practicum II:

Methods for analysis of cell mediated immunity

Flow cytometry - principle of the method. Phenotypization of the cells - application in different areas. Analysis of the activation state of leucocytes, thrombocytes and other cells.

Functional tests of lymphocytes - cytotoxicity, proliferation, cytokine secretion

Functional tests of phagocyting cells - ingestion, metabolic burst of Neu

Practical demonstration: analysis of membrane markers of peripheral blood lymphocytes

Based on case studies

Study Objective:

The goal of the Course is to get knowledge for understanding of pathofysiological characteristics of abnormal immune reactions, to get the competence to explain mechanisms of immune mediated diseases.

Study materials:

Key literature:

Roitt I, Delves PJ: Essential Immunology (Blackwell Scientific Publications

2001, 10th ed)

Recommended literature:

Kuby J: Immunology, New York : W.H. Freeman, 1992

Immunology-10th ed. - Blackwell Scientific Publications, 2001.

Immunology-10th ed. - Blackwell Scientific Publications, 2001.

Note:

Further information:

No time-table has been prepared for this course

The course is a part of the following study plans: