Clinical Laboratory Instrumentation
- Grading of the course requires grading of the following courses:
- Biochemistry (17ABBBCH)
Physical Chemistry (17ABBFCH)
- Garant předmětu:
- Martina Turchichová
- Stanislav Gajdoš, Iveta Horáčková, Martina Turchichová
- Kateřina Dunovská, Stanislav Gajdoš, Iveta Horáčková, Martina Turchichová
- Department of Biomedical Technology
Clinical laboratory instrumentation introduces principles of bioanalytical methods used in clinical diagnostics. Emphasis is put on optical methods (UV-VIS spectrophotometry, IR spectroscopy, AAS, AES, fluorimetry), NMR and X-ray analysis, electrochemical and electromigration methods (ion electrodes, biosensors, electrophoresis, isoelectric focusing), imunoassays and genetic methods (ELISA, PCR) as well as on chromatography and mass spectrometry. Contribution of lab automation to clinical diagnostics will be also discussed.
During the laboratory course students will be introduced into the basics of work in bioanalytical laboratory and lab data processing.
Mandatory attendance at all tutorials. Students have to come in time and be
well prepared. Absences are acceptable for serious reasons only, have to be
apologized and recompensed.
At every entrance-test, students have to score at least 60% of total points
to be admitted to the tutorial; if not, the tutorial (including the test)
will be recompensed on another date. A maximum of one tutorials can be
recompensed in total.
Working out all assigned exercises and submitting laboratory reports in
Successfully passing the credit-test.
Students have to pass a written test comprising the topics discussed during
both lectures and tutorials. The exam is graded according to the ECTS
- Syllabus of lectures:
1. Princles of bioanalytical methods and clinical diagnostic
2. Optical methods I
3. Optical methods II
4. Electrochemical methods
5. Electrochemical methods for measuring blood gases
6. Biosensors and automation
7. Electromigration methods
8. Genetic techniques
9. Imunochemical methods
10. Membrane processes
11. Centrifugation and extraction
12. Chromatography I
13. Chromatography II
14. Mass spectrometry
- Syllabus of tutorials:
1. Laboratory safety rules
2. UV-VIS spectrophotometry
3. Potenciometric titration of aminoacids
4. Agar elfo
5. Measurement of concentration/partial pressure of CO2 with gas probe, Measurement of partial pressure of O2 with galvanic probe
6. Gel chromatography
7. Simulation fo HIV detection by ELISA
- Study Objective:
Goal of the study is to get to know the principles of common bioanalytical methods used in a clinical laboratory, to work with scientific text and to discuss the current topics, and mainly, to solve model laboratory problems and tasks, which may happen in clinical diagnostics.
- Study materials:
1. Kenkel, John. Analytical chemistry for technicians. 4th ed. Boca Raton: CRC Press/Taylor & Francis, 2014. ISBN 978-1-4398-8105-7
2. Mikkelsen, S. R., Cortón, E.: Bioanalytical Chemistry. Wiley & Sons, Inc. Hoboken 2004.
3. Arneson, W., Brickell, J.: Clinical Chemistry - A Laboratory Perspective. F. A. Davis Company, Philadelphia 2007.
4. Gauglitz, G., Vo-Dinh, T.: Handbook of Spectroscopy. Wiley & Sons, Inc. Hoboken 2003.
5. Günzler, H., Williams, A.: Handbook of Analytical Techniques. Wiley & Sons, Inc. Hoboken 2001.
6. Nelson, D. L., Cox, M. M.: Lehninger principles of biochemistry. W. H. Freeman, New York 2008.
7. Voet, D., Voet, J. G.: Biochemistry. Wiley & Sons, Inc. Hoboken 2004.
- Time-table for winter semester 2022/2023:
- Time-table is not available yet
- Time-table for summer semester 2022/2023:
Lab. chemie a bioanalyt. metod
- The course is a part of the following study plans:
- Biomedical Technician - full time study in English (compulsory course)