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CZECH TECHNICAL UNIVERSITY IN PRAGUE
STUDY PLANS
2023/2024

Clinical Laboratory Instrumentation

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Code Completion Credits Range Language
17ABBLT Z,ZK 4 2P+2L English

The course 17ABBLT can be graded only after the course 17ABBFCH has been successfully completed.

The course 17ABBLT can be graded only after the course 17ABBBCH has been successfully completed.

Garant předmětu:
Martina Turchichová
Lecturer:
Stanislav Gajdoš, Iveta Horáčková, Martina Turchichová
Tutor:
Kateřina Dunovská, Stanislav Gajdoš, Iveta Horáčková, Martina Turchichová
Supervisor:
Department of Biomedical Technology
Synopsis:

Clinical laboratory instrumentation introduces principles of bioanalytical methods used in clinical diagnostics. Emphasis is put on optical methods (UV-VIS spectrophotometry, IR spectroscopy, AAS, AES, fluorimetry), NMR and X-ray analysis, electrochemical and electromigration methods (ion electrodes, biosensors, electrophoresis, isoelectric focusing), imunoassays and genetic methods (ELISA, PCR) as well as on chromatography and mass spectrometry. Contribution of lab automation to clinical diagnostics will be also discussed.

During the laboratory course students will be introduced into the basics of work in bioanalytical laboratory and lab data processing.

Requirements:

Credit requirements:

Mandatory attendance at all tutorials. Students have to come in time and be

well prepared. Absences are acceptable for serious reasons only, have to be

apologized and recompensed.

At every entrance-test, students have to score at least 60% of total points

to be admitted to the tutorial; if not, the tutorial (including the test)

will be recompensed on another date. A maximum of one tutorials can be

recompensed in total.

Working out all assigned exercises and submitting laboratory reports in

satisfying quality.

Successfully passing the credit-test.

Final examination:

Students have to pass a written test comprising the topics discussed during

both lectures and tutorials. The exam is graded according to the ECTS

scale.

Syllabus of lectures:

1. Princles of bioanalytical methods and clinical diagnostic

2. Optical methods I

3. Optical methods II

4. Electrochemical methods

5. Electrochemical methods for measuring blood gases

6. Biosensors and automation

7. Electromigration methods

8. Genetic techniques

9. Imunochemical methods

10. Membrane processes

11. Centrifugation and extraction

12. Chromatography I

13. Chromatography II

14. Mass spectrometry

Syllabus of tutorials:

1. Laboratory safety rules

2. UV-VIS spectrophotometry

3. Potenciometric titration of aminoacids

4. Agar elfo

5. Measurement of concentration/partial pressure of CO2 with gas probe, Measurement of partial pressure of O2 with galvanic probe

6. Gel chromatography

7. Simulation fo HIV detection by ELISA

Study Objective:

Goal of the study is to get to know the principles of common bioanalytical methods used in a clinical laboratory, to work with scientific text and to discuss the current topics, and mainly, to solve model laboratory problems and tasks, which may happen in clinical diagnostics.

Study materials:

1. Kenkel, John. Analytical chemistry for technicians. 4th ed. Boca Raton: CRC Press/Taylor & Francis, 2014. ISBN 978-1-4398-8105-7

2. Mikkelsen, S. R., Cortón, E.: Bioanalytical Chemistry. Wiley & Sons, Inc. Hoboken 2004.

3. Arneson, W., Brickell, J.: Clinical Chemistry - A Laboratory Perspective. F. A. Davis Company, Philadelphia 2007.

4. Gauglitz, G., Vo-Dinh, T.: Handbook of Spectroscopy. Wiley & Sons, Inc. Hoboken 2003.

5. Günzler, H., Williams, A.: Handbook of Analytical Techniques. Wiley & Sons, Inc. Hoboken 2001.

6. Nelson, D. L., Cox, M. M.: Lehninger principles of biochemistry. W. H. Freeman, New York 2008.

7. Voet, D., Voet, J. G.: Biochemistry. Wiley & Sons, Inc. Hoboken 2004.

Note:
Time-table for winter semester 2023/2024:
Time-table is not available yet
Time-table for summer semester 2023/2024:
06:00–08:0008:00–10:0010:00–12:0012:00–14:0014:00–16:0016:00–18:0018:00–20:0020:00–22:0022:00–24:00
Mon
Tue
roomKL:A-007
Dunovská K.
08:00–11:50
EVEN WEEK

(lecture parallel1)
Kladno FBMI
Lab. chemie a bioanalyt. metod
Wed
Thu
Fri
roomKL:B-230
Turchichová M.
08:00–09:50
(lecture parallel1)
Kladno FBMI
Učebna
The course is a part of the following study plans:
Data valid to 2024-04-11
Aktualizace výše uvedených informací naleznete na adrese https://bilakniha.cvut.cz/en/predmet2178706.html