Practical Exercises in Microbiology

Course guarantor:

Lecturer:

Tutor:

Supervisor:

Department of Nuclear Chemistry

Synopsis:

The first part of practical exercises is devoted to the principles of microbiological work, cultivation of micro-organisms, morphology and cytology of micro-organisms. In the the next part, the students observe the influence of external conditions on micro-organisms and they learn to isolate the micro-organisms as well as to evaluate of cell count in environment. Finaly, the attention is devoted to study of micro-organism growth, fundamental genetics preparations, host strains of Escherichia coli and identification of micro-organisms.

Requirements:

Fundamental knowledge of biochemistry

Syllabus of lectures:

Syllabus of tutorials:

1. Safety precautions in microbiological laboratory. Preparation of ster

sterile instruments. Defined and complex media. Preparation and sterilization

of various cultivation and diagnostic media. Methods for inoculation of selected

types of germs and yeasts. Light microscopy of untreated and dyed samples of

germs and yeasts. Dying according to Gram for selected germ strains.

2. Methods of micro-organisms isolation. Isolation of pure strains from

from mixture. Microscopy of yeasts - types of vegetative propagation of yeasts.

Colouring of yeasts with methylene blue. Methods of yeast counting in counting

chamber.

3. Factors affecting germ growth. Influence of temperature, organic dyes

dyes, UV and antibiotics on growth and propagation of micro-organisms.

Principles of germ count determination in food. Introduction to ISO.

4. Identification of isolated germ and yeast strains. Biochemical test

tests of isolated samples. Use of ENTERO-test for their identification.

Introduction to moulds morphology. Use of microscope for classification of

common moulds. Principles of system and identification of moulds.

5. Growth curve of micro-organisms. Calculation of growth rate from expe

experimental data - cultivation of E. coli, determination of optical density.

Determination of growth rate, lag phase and generation time.

6. Modern fast methods for identification. Removal of all used strains and

and media, washing of used chemical glass. Exam test. Evaluation of knowledge

and results of work. Credit.

Study Objective:

To acquaint the students with a basic practice in microbiology lab

Study materials:

Key reference:

K. Demnerová et al.:Laboratory course in microbiology, skripta VŠCHT, 1998

Recommended reference:

T. R. Johnson, Ch. L. Case: Laboratory Experiments in Microbiology, 8. Edition,

Benjamin Cummings, March 2006

Note:

Further information:

No time-table has been prepared for this course

The course is a part of the following study plans: