Selected Topics of Solid State Structure

Garant předmětu:

Lecturer:

Tutor:

Supervisor:

Department of Solid State Engineering

Synopsis:

The lecture cycle focuses on the structure of solids from the point of view of the arrangement of atoms. The first part focuses on application-interesting structures from metallic materials to molecular crystals. In the second part we will look at the possibilities of observing the atomic structure using X-rays, both from the point of view of the average and the local structure. Objective of the course is also the use of special programs designed to study and analyze the structure and microstructure of solids.

Requirements:

Syllabus of lectures:

1. Introduction to physical methods of structural biology. 2. Structure and properties of proteins and nucleic acids. 3. Crystallization methods. 4. Single crystal diffraction of proteins and nucleic acids. 5. Overcoming the phase problem in biological structures. 6. Structure refinement and validation; structure-function relationship and biological significance. 7. Databases of macromolecular structures.

Syllabus of tutorials:

1. Protein sample characterisation. 2. Protein crystallisation. 3. Single crystal diffraction experiment. 4. Diffraction data processing. 5. Model building and refinement using 3D graphics. 6. Structure analysis of a bacterial or viral protein.

Study Objective:

Knowledge

Students with knowledge of mathematics and physics on Bc. level should attend. Some preliminary knowledge of crystallography is an advantage.

Skills

In the course students will aquire the knowledge and skills in methods of study of 3D structure of biological molecules, practical appliaction of protein crystallography, and ability to apply the method in drug design or biotechnologies.

Study materials:

"Povinná literatura:

R. E. Dinnebier, S.J.L. Billinge editors: Powder Diffraction: Theory and Practice, RSC Publishing, ISBN: 978-0-85404-231-9, Cambridge 2008.

Doporučená literatura:

T. Egami, S.J.L. Billinge : Underneath the Bragg Peaks – Structure analysis of complex materials, 2 nd edition, Elsevier, ISBN: 978-0-08-097133-9, Amsterdam 2013.

M. De Graff, M. E. McHencry: Structure of Materials: An Introduction to Diffraction, and Symmetry, Cambridge university press, ISBN: 978-0-521-65151-6, Cambridge 2008.

J. Marek, Z. Trávníček: Monokrystalová rentgenová strukturní analýza, Universita Palackého v Olomouci, ISBN: 80-244-0551-2, Olomouc 2002

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Note:

Further information:

No time-table has been prepared for this course

The course is a part of the following study plans:

• Inženýrství pevných látek (elective course)