Cellular and Molecular Diagnostics

Garant předmětu:

Lecturer:

Tutor:

Supervisor:

Department of Natural Sciences

Synopsis:

The students will obtain information regarding basic diagnostic methods used in cell biology with particular focus on laboratory training.

Requirements:

Basic (university) knowledge of cellular and molecular biology

Syllabus of lectures:

Theory:

1.Cell, multicellular organism, microenvironment

2.Intracellular signal transduction

3.Laboratory models in vitro and in vivo

4.Methods for assesments of cell viability, proliferation, and death

5.Methods for analyses of surface and cytoplasmic markers

6.Tumor antigens and cytokine production

Syllabus of tutorials:

Laboratory training:

1.Detection of cell prosperity and plasma membrane permeability

Tasks: Cell counting, WST-1 assay, LDH assay

2.2D a 3D tumor model development

Task: Tissue cultures

3.Specific cells detection within tumor microenvironment using fluorescent microscopy or flow cytometry

Task: Surface and cytoplasmic antigens staining

Study Objective:

We will introduce students to experimental techniques used in cell biology laboratories and obtaining basic laboratory practice.

Study materials:

Basic literature:

Václav Hořejší, Jiřina Bartůňková: Základy imunologie. Triton, 4. vyd., 2009

Oldřich Nečas: Obecná biologie pro lékařské fakulty. 1. vyd. Jinočany: H&H, 2000.

Recommended literature:

Donald Voet, Judith G. Voet: Biochemie. Victoria Publishing 2005.

Note:

Further information:

No time-table has been prepared for this course

The course is a part of the following study plans:

• Nanotechnology (compulsory course)