Instrumental Methods 1

Relations:

In order to register for course 15PINS, the student must have registered for course 15PINS in a previous semester or in the same semester.

Course guarantor:

Lecturer:

Martin Vlk, Alena Zavadilová

Tutor:

Supervisor:

Department of Nuclear Chemistry

Synopsis:

Overview of selected modern instrumental methods of research and analysis, theoretical fundamentals, instrumental technique, utilization and application.

Requirements:

The course is completed with an examination, which consists of a written and an oral part taking place on the same day. A prerequisite for admission to the oral examination is the successful completion of the written part. The oral part is graded. All questions are drawn at random, and preparation time is provided. In the case of a grade F, both parts of the examination must be retaken.

During the first half of the semester, only lectures take place in the INSN 1 and PINS time slots, while in the second half of the summer semester, only practical classes are held in both time slots.

Syllabus of lectures:

Overview of basic types of radiation sources and detectors used in basic techniques of analytical methods, principles of these methods, introduction to selected advanced instrumental methods of research and analysis, utilization and application.

Outline:

1.Overview of basic radiation sources and principles for X-ray, UV-VIS, IR

2.Overview of basic detectors and principles of detection

3.Basics of optics and spectroscopy

4.Basics of vacuum technology (sources, measurements)

5.Selected analytical methods

UV-VIS absorption spectrometry

Atomic absorption spectrometry

Infrared absorption spectroscopy (basic FTIR techniques)

Absorption methods on beams of synchrotron radiation

Raman spectroscopy

Methods based on emission of radiation (Luminescent spectrometry (TRLFS, LIBS, etc.), X-ray photoelectron spectroscopy)

Mössbauer spectroscopy

Nuclear magnetic and paramagnetic resonance

Chromatographic analytical methods (High performance liquid chromatography, Gas chromatography)

Mass spectrometry (MS)

Thermal analysis methods (eg differential thermal analysis, thermogravimetry, DSC, TD, EGA, ETA)

Modern microscopy ( electron microscopy, fluorescence microscopy (SIM, STED, STORM, etc.)

Syllabus of tutorials:

Study Objective:

Make the acquaintance of modern instrumental methods of research and analysis.

Study materials:

Povinná literatura:

H. Gunzler et. al.: Handbook of Analytical Technichniques, Willey-VCH Verlag GmbH, Weinheim 2002.

Naito A.: Experiemntal Approaches of NMR Spectroscopy. Methodology and Application to Life Science and Materials Science. Springer 2018

Y. Engelborghs, A. J.W.G. Visser (Eds.): Fluorescence Spectroscopy and Microscopy, Humana Press, 2014

Další doporučená literatura:

J.H. Gross: Mass Spectrometry. Springer 2017.

Note:

Time-table for winter semester 2025/2026:

Time-table is not available yet

Time-table for summer semester 2025/2026:

Time-table is not available yet

The course is a part of the following study plans:

• Jaderná chemie (compulsory course in the program)