Microbiology and Imunology

Garant předmětu:

Lecturer:

Tutor:

Supervisor:

Department of Natural Sciences

Synopsis:

Microbiology: Organization and function in microbial cell. Procaryotic cell, binary fission. Phylogenetic system of bacteria and archea. Cyanobacteria. Microbial nutrition, microbial growth - life cycle of procaryotic cell, growth curve, kinetics of bacterial growth. Influence of environmental factors on microbial growth - temperature, pH, extreme environment. Antimicrobial, antibacterial agents - antibiotics and mechanisms of their effect, dezinfection and sterilization. Energetic metabolism. Genetics and molecular biology of microorganisms. Microflora of human body. Microbial diseases and their control.

Immunology: Cells and organs of the immune system. Antigens. Immune reaction. Main histocompatibility complex. B-lymphocytes. Production of immunoglobulins. Immunoglobulins, function and structure. T-lymphocytes and cellular immunity. Cytotoxic lymphocytes. Cytokines. Immunity against infection. Non-specific immunity. Allergic diseases. Immunity and nutrition. Immunopathology. Immunodeficiency. Autoimmune diseases.

Requirements:

Assessment: laboratory practices elaboration, succesful mastery of written work.

Examination: class ticket, succesful mastery of written work

Syllabus of lectures:

1.Microbiology: Organization and function in microbial cell. Procaryotic cell, binary fission. Phylogenetic system of bacteria and archea. Cyanobacteria.

2.Microbial nutrition, microbial growth - life cycle of procaryotic cell, growth curve, kinetics of bacterial growth.

3.Influence of environmental factors on microbial growth - temperature, pH, extreme environment. Antimicrobial, antibacterial agents - antibiotics and mechanisms of their effect, dezinfection and sterilization.

4.Energetic metabolism. Genetics and molecular biology of microorganisms.

5.Microflora of human body.

6.Microbial diseases and their control.

7.Immunology: Cells and organs of the immune system.

8.Antigens.

9.Immune reaction.

10.Main histocompatibility complex. B-lymphocytes. Production of immunoglobulins. Immunoglobulins, function and structure. T-lymphocytes and cellular immunity. Cytotoxic lymphocytes. Cytokines.

11.Immunity against infection. Non-specific immunity. Allergic diseases. Immunity and nutrition.

12.Immunopathology. Immunodeficiency. Autoimmune diseases.

Syllabus of tutorials:

1. Sterilization and desinfection methods.

2. Basic praxis in microbiological laboratory.

3. Bacterial DNA isolation.

4. Bacterial cultivation.

5. Gram staining.

6. Imonogenetics - excercices

Study Objective:

Micro-organism, microflora of human body, microbial disease

Study materials:

1.NORTHROP, Robert B. a Anne N. CONNOR. Introduction to molecular biology, genomics, and proteomics for biomedical engineers. Boca Raton: CRC Press/Taylor & Francis, 2009.

2.ALBERTS, Bruce et al. Molecular biology of the cell: reference edition. 5th ed. New York: Garland Science, 2008.

3.LODISH, Harvey F. et al. Molecular cell biology. 6th ed. New York: W. H. Freeman, 2008.

4.Robert L. Nussbaum, Roderick R. McInnes, Huntington F.Willard:Thompson and Thompson genetics in medicine, W.B. Saunders, Philadelphia 2001, USA, ISBN 0-7216-6902-6

5.KŘENOVÁ, Drahomíra a Berta OTOVÁ. How to practise biological and medical genetics. 1st ed. Prague: Karolinum, 2003.

Note:

Further information:

No time-table has been prepared for this course

The course is a part of the following study plans:

• Optics and Optometry - full-time (compulsory course)