Analytical Chemistry 2

The course is not on the list Without time-table
Code Completion Credits Range
15ANL2 ZK 4 3+0
Garant předmětu:
Department of Nuclear Chemistry

Analytical chemistry II is the continuation of lecture Analytical chemistry I. This course is oriented to the instrumental methods of analysis and processing of analytical results.


Knowledge of general chemistry on the level of a completed basic university course. Completed course „Analytical Chemistry I“.

Syllabus of lectures:

1. Absolute and comparative analytical methods. Calibration methods

(calibration curve method, standard addition method).

2. Basic statistical evaluation of data (accuracy and precision, mean, standard

deviation, confidence interval, valid numbers).

3. Electroanalytical methods based on measurement of potential. Equilibrium

potentiometry (electrochemical cell, electrodes). Direct potentiometry

(measurement of pH, determination of other ions using ISE, selectivity

coefficient, gas sensors based on ISE). Indirect potentiometry, potentiometric


4. Electroanalytical methods based on measurement of current I. Voltammetry and

polarography (polarization curve, scheme of electrochemical cell, electrodes,

dc, ac and pulse voltammetry. Voltammetric stripping analysys. Amperometry

(amperometric detectors).

5. Electroanalytical methods based on measurement of current II.

Electrogravimetry and coulometry (potentiostatic and galvanostatic methods,

coulometric titrations, colometric analyzers). Conductometry (conductometric

analyzers and detectors).

6. Optical methods (the nature of absorption and emission of radiation by atoms

and molecules). Atomic and molecular absorption and emission/fluorescence

spectra, their utilization in analytical chemistry. Molecular absorption

spectrometry in uv/vis range of radiation. Atomic absorption spectrometry.

7. Automatic chemical analysers (FIA, CFA). Nonspectral optical methods

(refractometry, polarimetry, nephelometry).

8. Chromatographic methods (fundamentals, classification of chromatographic

methods). Planar chromatography. Column chromatography (general aspects,

parameters obtained from chromatogram, separation efficiency, resolution,

optimization of separation process). Application of chromatographic methods in

quantitative and qualitative analysis (hyphenated methods, GC-MS, LC-MS).

9. Gas chromatography (columns, detectors). Liquid chromatography (selection of

mobile phase, classification of LC based on difference in stationary phase -

partition, affinity, size-exclusion, ion-exchange chromatography).

10. Electromigration methods. Separation in electric field (zone and capillary

electrophoresis). Example of separation - aminoacids and peptides.

Syllabus of tutorials:
Study Objective:

The course of analytical chemistry provides the students with the knowledge of the basic methods of analytical chemistry and schemes of analytical procedures.

The students will acquire competence to select independenty optimum analytical approach to the given problem.

Study materials:

Key literature:

1. D.A.Skoog, D.M.West and F.J.Holler, Fundamentals of Analytical Chemistry, 6.

Edition, Saunders College Publishing, 1992;

Recommended literature:

1. Kellner R., Mermet J.M., Otto M., Widmer H.M. (Editors): Analytical

Chemistry, Willey-VCH, Weinheim 1998 (ISBN 3-527-28610-1).

Further information:
No time-table has been prepared for this course
The course is a part of the following study plans:
Data valid to 2024-06-15
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