Clinical Laboratory Instrumentation

Grading of the course requires grading of the following courses:

Biochemistry (17PBBBCH)
Physical Chemistry (17PBBFCH)

Garant předmětu:

Lecturer:

Tutor:

Supervisor:

Department of Biomedical Technology

Synopsis:

Clinical laboratory instrumentation introduces principles of bioanalytical methods used in clinical diagnostics. Emphasis is put on optical methods (UV-VIS spectrophotometry, IR spectroscopy, AAS, AES, fluorimetry), NMR and X-ray analysis, electrochemical and electromigration methods (ion electrodes, biosensors, electrophoresis, isoelectric focusing), imunoassays and genetic methods (ELISA, PCR) as well as on chromatography and mass spectrometry. Contribution of lab automation to clinical diagnostics will be also discussed.

During the laboratory course students will be introduced into the basics of work in bioanalytical laboratory and lab data processing.

Requirements:

Credit requirements:

Mandatory attendance at all tutorials. Students have to come in time and be

well prepared. Absences are acceptable for serious reasons only, have to be

apologized and recompensed.

At every entrance-test, students have to score at least 60% of total points

to be admitted to the tutorial; if not, the tutorial (including the test)

will be recompensed on another date. A maximum of two tutorials can be

recompensed in total.

Working out all assigned exercises and submitting laboratory reports in

satisfying quality.

Successfully passing the credit-test.

Final examination:

Students have to pass a written test comprising the topics discussed during

both lectures and tutorials. The exam is graded according to the ECTS

scale.

Syllabus of lectures:

1. Princles of bioanalytical methods and clinical diagnostic

2. Optical methods I

3. Optical methods II

4. Electrochemical methods (galvanic sond for dissolved oxygen measurement, alternatives for Clark probe, advantages and disadvantages of its use, methods of calibration)

5. Electrochemical methods for measuring blood gases (Astrup, instrumentation of methodd, used electrodes - its type and construction, calculation of potentials of electrodes, gas CO2 probe - specifics of measurement, factors affectig measurement, right experimantal conditions setup)

6. Biosensors and automation

7. Electromigration methods

8. Genetic techniques

9. Imunochemical methods

10. Membrane processes

11. Centrifugation and extraction

12. Chromatography I

13. Chromatography II

14. Massí spectrometry

Syllabus of tutorials:

1. Laboratory safety rules. Buffers preparation, principles of statistical evaluation of experimental results

2. Spectrophotometric evaluation of protein concentration.

3. Acidobasic titration of aminoacids. Contuctivity of ultrapure water

4. Agarose gel electrophoresis

5. Electrochemical techniques of measurement dissolved gases (Measurement of concentration/partial pressure of CO2 with gas probe, Measurement of partial pressure of O2 with galvanic probe)

6. Gel chromatography

7. Simulation of HIV detection by ELISA

Study Objective:

Goal of the study is to get to know the principles of common bioanalytical methods used in a clinical laboratory, to work with scientific text and to discuss the current topics, and mainly, to solve model laboratory problems and tasks, which may happen in clinical diagnostics.

Study materials:

1. Kenkel, John. Analytical chemistry for technicians. 4th ed. Boca Raton: CRC Press/Taylor & Francis, 2014. ISBN 978-1-4398-8105-7

2. Mikkelsen, S. R., Cortón, E.: Bioanalytical Chemistry. Wiley & Sons, Inc. Hoboken 2004.

3. Arneson, W., Brickell, J.: Clinical Chemistry - A Laboratory Perspective. F. A. Davis Company, Philadelphia 2007.

4. Gauglitz, G., Vo-Dinh, T.: Handbook of Spectroscopy. Wiley & Sons, Inc. Hoboken 2003.

5. Günzler, H., Williams, A.: Handbook of Analytical Techniques. Wiley & Sons, Inc. Hoboken 2001.

6. Nelson, D. L., Cox, M. M.: Lehninger principles of biochemistry. W. H. Freeman, New York 2008.

7. Voet, D., Voet, J. G.: Biochemistry. Wiley & Sons, Inc. Hoboken 2004.

Note:

Further information:

No time-table has been prepared for this course

The course is a part of the following study plans:

• Biomedical Technician - full time study (compulsory course)