Fluorescence Spectroscopy in Biology & Medicine

Garant předmětu:

Lecturer:

Tutor:

Supervisor:

Department of Natural Sciences

Synopsis:

In the first and largest part the principles and approaches of modern

fluorescence spectroscopy will be discussed. Each specific approach based on

fluorescence spectroscopy will be illustrated on biological applications. The

second part is devoted to dyes used in biological fluorescence spectroscopy. The

third part is dealing with fluorescence microscopy and single molecule

techniques. Again biological and medical relevant applications of those

techniques will be discussed. The last lecture is dealing with Photodynamic

Therapy, which is indeed a fluorescence application in medicine.

Requirements:

Syllabus of lectures:

1. Absorption of light and electronic transitions

2. Principles and histrory of fluorescence

3. Fluorescence Lifetime and its biological application

4. Fluoresccence Anisotropy and its biological applications

5. Fluoresccence Quenching and its biological applications

6. Solvent relaxation method and its application in biological sciences

7. Theodor Foerster: Resonance energy transfer and excimer formation

8. Fluorophores and Labelling

9. Green Fluorescent Protein and its applications

10. Confocal and two-photon microscopy

11. Principles of Fluorescence Correlation Spectroscopy (FCS) and its

application in biomembrane research

12. Application of FCS in development of carriers for non-viral gene therapy

13. Photodynamic Therapy

Syllabus of tutorials:

Study Objective:

In the first and largest part the principles and approaches of modern

fluorescence spectroscopy will be discussed. Each specific approach based on

fluorescence spectroscopy will be illustrated on biological applications. The

second part is devoted to dyes used in biological fluorescence spectroscopy. The

third part is dealing with fluorescence microscopy and single molecule

techniques. Again biological and medical relevant applications of those

techniques will be discussed. The last lecture is dealing with Photodynamic

Therapy, which is indeed a fluorescence application in medicine.

Study materials:

[1] Hof M., Hutterer R., Fidler V.: Fluorescence Spectroscopy in Biology.

Springer Verlag

Gauglitz G., Vo-Dinh T.: Handbook of Spectroscopy. Wiley VCH Verlag, Weinheim

2003, Heidelberg 2004

[2] Lakowicz J.R.: Principles of Fluorescence Spectroscopy, 3rd edn. Springer

2006

Note:

Further information:

No time-table has been prepared for this course

The course is a part of the following study plans: