Laboratory Medical Equipment
- Department of Biomedical Technology
The subject follows the previous knowledge of the students of general chemistry and biochemistry and presents them the methods of work and instrumentation in the biochemical and clinical laboratory. Students will be introduced into principles of individual methods, their applications in clinical biochemistry and their technical aspects. Students will be acquainted with new trends in medical determinations, such as immunoassay, mass spectrometry and POCT determination.
During the laboratory exercises the students will be introduced to the laboratory equipment of the bioanalytical and clinical laboratories, will get acquainted with the specifics of the laboratory analysis of the biological material and with the correct principles of laboratory data processing.
Requirements for assesment:
Compulsory attendance at tutorials, absence must be properly apologized (for serious reasons) and recompensed.
Passing entrance test to tutorial at least to 50%. If the number of points is smaller, the exercise must be replaced, including test. In total, a maximum of two exercises can be substituted.
Write and submit of all protocols from tutorials.
Passing the written test at the end of the semester. The written test covers the lectures and exercises topics. The test must be written at least to 50%.
- Syllabus of lectures:
1. Basic conception of laboratory equipment
2. Chromatography and centrifugation
3. Mass spectrometry
4. Electromigration methods
5. Electrochemical methods and optical methods
6. Imunochemistry and genetic methods
7. Automatisation,maitenance and measuring of POCT
- Syllabus of tutorials:
1. Gel chromatography
2. Evaluation of plasma proteins, agarose electrophoresis
3. Carbohydrates properties and their evaluation in clinical laboratory
4. Mass spectrometry
5. Measurement of concentration/partial pressure of CO2 with gas probe
6. Measurement of partial pressure of O2 with galvanic probe
7. Simulation of HIV detection by ELISA
- Study Objective:
Main goal of study is to present bioanalytical method and its application used in medical equipment and to understand the specific requirements for work with biological samples in laboratories. Students should be familiar with work according to protocol and creating of the protocols and processing of their laboratory results.
- Study materials:
1. Mikkelsen, S. R., Cortón, E.: Bioanalytical Chemistry. Wiley &amp; Sons, Inc. Hoboken 2004.
2. Arneson, W., Brickell, J.: Clinical Chemistry - A Laboratory Perspective. F. A. Davis Company, Philadelphia 2007.
3. Gauglitz, G., Vo-Dinh, T.: Handbook of Spectroscopy. Wiley &amp; Sons, Inc. Hoboken 2003.
4. Günzler, H., Williams, A.: Handbook of Analytical Techniques. Wiley &amp; Sons, Inc. Hoboken 2001.
5. Nelson, D. L., Cox, M. M.: Lehninger principles of biochemistry. W. H. Freeman, New York 2008.
6. Voet, D., Voet, J. G.: Biochemistry. Wiley &amp; Sons, Inc. Hoboken 2004.
- Further information:
- No time-table has been prepared for this course
- The course is a part of the following study plans:
- Medical Laboratory Technician - full-time study (compulsory course)