Clinical Biochemistry and Laboratory Diagnostic Methods
- Department of Biomedical Technology
Course participants will be introduced to the biochemistry of the human organism, with important metabolic and regulatory pathways and disorders of these processes. Emphasis is placed on the possibilities of diagnosing these disorders and the procedures of the relevant laboratory examinations. This subject connects and completes the knowledge gained during the study of previous chemical disciplines.
Within the tutorials, the students will be introduced closer to the clinical laboratory and will also deal with data processing from the methods used in clinical laboratories. The topics discussed at the lectures will be more closely discussed and expanded.
Requirements for assignment:
Compulsory attendance at all tutorials, absence must be properly apologized (for serious reasons) and replaced. In total, a maximum of three exercises can be substituted.
Completing all task given during tutorials
Submitting of protocols from data processing tutorials
Presentation on a given topic
The exam consists of a written test and covers the topics of all lectures and exercises.
The overall assessment of the test corresponds to the valid ECTS grading scale.
Upon successful completion of the written test, the oral examination can be provided for exam grade improvement.
More detailed information in the „Demands for passing the subject“ document.
- Syllabus of lectures:
1. Introduction to clinical biochemistry, preanalytics and properties of an analytical method
2. Composition of organisms, main analytes and suitable diagnostic methods
3. Principles of metabolism and organism integration
4. Metabolism of carbohydrates and the diagnostics of its disorders
5. Metabolism of lipids and the diagnostics of its disorders
6. Metabolism of nitrogen compounds (amino acids, purines and nucleotides) and the diagnostics of its disorders
7. Blood, proteins of blood plasma, diagnostics of inflammation and cardiovascular diseases
8. Enzymes and estimation of its activity, immunity
9. Hormones and neurotransmiters, their estimation and clinical estimation during pregnancy
10. Clinical biochemistry of inner environment, water and ion management, acid-base equilibrium
11. Nutrition and digestion, biochemical diagnostics of gastroenterology
12. Biochemistry of liver, clinical diagnostics in hepatology
13. Function of kidney and urethra and diagnostics of theur disorders
14. Basics of toxicology
- Syllabus of tutorials:
1. Laboratory safety rules
2. Preanalytical errors. Preparation of solutions (lab)
3. Properties of human body compartements. Principles of diagnostic methods (revision of topics)
4. Basic principles and connections of metabolism (revision of topics)
5. Carbohydrates properties and their evaluation in clinical laboratory (lab)
6. Properties of cholesterol, phospholipids, bile and lipoprotein particles (lab)
7. Evaluation of plasma proteins, agarose electrophoresis. (lab)
8. Digestive enzymes, evaluation of enzyme aktivity of lactatedehydrogenase (lab)
9. Gel chromatography(lab)
10. Measurement of concentration/partial pressure of CO2 with gas probe, (lab)
11. Measurement of partial pressure of O2 with galvanic probe (lab)
12. Simulation of HIV detection by ELISA (lab)
13. Liver functions and diseases (lab)
14. Biochemical evaluation of urine (pH, proteins, hemoglobine and ketobodies) (lab)
- Study Objective:
The aim of the study is to understand the methods of laboratory diagnostics, to understand the basic principles of human metabolism, its regulation and integration, and to learn how and why these disorders are manifested. Students will be introduced with the principles of laboratory examinations during the exercises. Another aim of the study is to learn how to work with scientific literature and present the studied knowledge to other students in the form of a presentation.
- Study materials:
 Murray, R.K., et al.: Harper´s illustrated biochemistry. Mc Graw Hill Medical, New York 2009.
 Nelson, D.L., Cox, M.M.: Lehninger principles of biochemistry, WH Freeman, New York 2008.
 Voet, D., Voet, J.G.: Biochemistry. Wiley, Hoboken 2011.
 Mikkelsen, S. R., Cortón, E.: Bioanalytical Chemistry. Wiley & Sons, Inc. Hoboken 2004.
- Further information:
- No time-table has been prepared for this course
- The course is a part of the following study plans:
- Biomedical Engineer - full-time study (compulsory course)