Biophotonics

Lecturer:

Miroslav Jelínek (gar.)

Tutor:

Jan Mikšovský (gar.), Miroslav Jelínek (gar.)

Supervisor:

Department of Natural Sciences

Synopsis:

Overview of principles and applications in interdisciplinary region connecting disciplines of physics, optics and biology. Interaction of optical radiation with matter, with tissue, basics of biology, photobiology, bioimaging, microscopy, basics of lasers, laser safety, optical biosensors, nanotechnology for biophotonics.

Requirements:

Assesment : active participation on the solution of exercises.

Examination : fulfilment of requirements for assesment, knowledge covering contect of the course.

Syllabus of lectures:

1) Biophotonics as scientific discipline

2) Interaction of radiation with matter I (dual character of light, photons,

coherence, interference, diffraction, bounds in molecules)

3) Basic of laser technique (laser principle, types of lasers, laser applications

in photobiology and in medicine, laser safety)

4) Basic of biology

5) Interaction of radiation with matter II. (interaction light - molecule,

interaction with matter, indication and detection of interaction mechanisms)

6) Absorption and emission of light, principles of biological spectroscopy

(absorption, emission, fluorescence, raman, Fourier infrared, etc.)

7) Bioimaging (principles, microscopy, types of microscopes - transmission,

fluorescence, tunneling, electron, etc.)

8) Optical biosensors (principles, contruction, applications), photomultipliers,

semiconductor detectors and sensors

9) Photodynamical therapy (principles, field of applications), cytometry

10) Interaction of radiation III. (interaction with tissue, regeneration,

phototherapy, optical coherence tomography)

11) Cells optical manipulation

12) Nanotechnology for biophotonic- bionanophotonic

13) Biomaterials for biophotonic

14) Conclusion, reserve

Syllabus of tutorials:

Excercises:

Microscopy of tissue.

Spectroscopy analysis of thin biocompatible films.

X-Ray tomography.

Interaction of optics irradiation with tissue.

Laboratory classes:

- Interaction of optics irradation with tissue

- Tension in thin films

- Young's modulus

- Wave theory of light

- Spectrometric measurements of concentration

- Study of photocatalytic surfaces.

Study Objective:

To present basic principles of photonics, basic principles of interaction of optical radiation with tissue and to explain frequent applications of photonics in medicine.

Overview of principles and applications in the interdisciplinary sphere, connecting physics, optics and biology. Interaction of laser radiation with matter, interaction of radiation with tissue, biology basics, photobiology, bioimaging, basics of lasers, laser safety, optical biosensors, photodynamical therapy, optical manipulation with cells, nanotechnology for biophotonics, biomaterials for photonics.

Study materials:

[1] Prasad P.N.: Introduction to Biophotonics. Wiley-InterScience 2004.

[2] Vymětalová V.: Laboratorní cvičení z biologie. VŠCHT Praha 2001.

[3] Základy fotoniky, svazek 1-4. Bahaa E. A. Saleh, Malvin Carl Teich. Vyd. MATFYZPRESS, 1994, Praha.

[4] Paras N. Prasad : Nanophotonics. John Wiley and Sons, Inc., 2004.

Note:

Time-table for winter semester 2011/2012:

	06:00–08:0008:00–10:0010:00–12:0012:00–14:0014:00–16:0016:00–18:0018:00–20:0020:00–22:0022:00–24:00
Mon
Tue
Fri	roomKL:GDM3 Jelínek M. 14:00–15:50 (lecture parallel1) Kladno FBMI Učebna GDM3_303
Thu
Fri	roomAL:LAS Jelínek M. 10:00–19:50 (lecture parallel1 parallel nr.101) Praha 2 - Albertov Albertov - laserová lab.

Time-table for summer semester 2011/2012:

Time-table is not available yet

The course is a part of the following study plans:

• Navazující magisterský studijní obor Přístroje a metody pro biomedicínu - prezenční (compulsory course)