Spectroscopy in biology and medicine

Lecturer:

Radek Macháň (gar.)

Tutor:

Radek Macháň (gar.)

Supervisor:

Department of Natural Sciences

Synopsis:

In the first and largest part the principles and approaches of modern

fluorescence spectroscopy will be discussed. Each specific approach based on

fluorescence spectroscopy will be illustrated on biological applications. The

second part is devoted to dyes used in biological fluorescence spectroscopy. The

third part is dealing with fluorescence microscopy and single molecule

techniques. Again biological and medical relevant applications of those

techniques will be discussed. The last lecture is dealing with Photodynamic

Therapy, which is indeed a fluorescence application in medicine.

Requirements:

A final test and optional later discussion in case of questionable outcome of the test. Further details will be anounced at the lessons

Syllabus of lectures:

1. Absorption of light and electronic transitions

2. Principles and history of fluorescence

3. Fluorescence lifetime and its biological applications

4. Fluorescence Quenching and its biological applications

5. Fluorescence Anisotropy and its biological applications

6. The effects of solvent on fluorescence spectra and their applications

7. Foerster resonance energy transfer

8. Green Fluorescent Protein and its applications

9. Fluorescence and chemical kinetics, excimer formation

10. Basic principles of fluorescence microscopy

11. Resolution in fluorescence microscopy and methods of its enhancement

12. Fluorescence correlation spectroscopy and its biological applications

13. Photodynamic Therapy

14. Laboratory excercise - emission spectra and fluorescence quenching

Syllabus of tutorials:

1. Lambert-Beer law

2. Jabloński diagram

3. monoexponencial fluorescence decay

4. Stern-Volmerova equation

5. Fluorescence polarisation, magic angle

6. Spectral shift in fluorescence as a function of polarity and temperature

7. Foerster resonance energy transfer

9. Fluorescence and chemical kinetics

10. Principles of optical microscopy - revision

11. Resolution in microscopy

12. Molecular diffusion

14. Laboratory excercise - emission spectra and fluorescence quenching

Study Objective:

To present principles of molecular fluorescence, fluorescence quenching and energy transfer and fluorescence spectroscopy techniques. Application of fluorescence spectroscopy in cell biology and medicine.

Study materials:

1] Hof M., Hutterer R., Fidler V.: Fluorescence Spectroscopy in Biology.

Springer Verlag

[2]Gauglitz G., Vo-Dinh T.: Handbook of Spectroscopy. Wiley VCH Verlag, Weinheim

2003, Heidelberg 2004

[3] Lakowicz J.R.: Principles of Fluorescence Spectroscopy, 3rd edn. Springer 2006

[4] Materials distributed during the course

Note:

Time-table for winter semester 2011/2012:

Time-table is not available yet

Time-table for summer semester 2011/2012:

	06:00–08:0008:00–10:0010:00–12:0012:00–14:0014:00–16:0016:00–18:0018:00–20:0020:00–22:0022:00–24:00
Mon
Tue
Fri
Thu	roomKL:B-309 Macháň R. 16:00–16:50 (lecture parallel1) Kladno FBMI zasedačkaroomKL:B-309 Macháň R. 17:00–17:50 (lecture parallel1 parallel nr.101) Kladno FBMI zasedačka
Fri

The course is a part of the following study plans:

• Navazující magisterský studijní obor Přístroje a metody pro biomedicínu - prezenční (compulsory elective course)