Immunochemistry

Lecturer:

Karel Bezouška (gar.)

Tutor:

Supervisor:

Department of Nuclear Chemistry

Synopsis:

The course is focused on the history and present of immunochemistry and molecular immunology. The most important molecules of immunity system are

thoroughly discussed (antibody, T-cell receptor, HLA antigens, complement, adhesive molecules) as well as technical aspects of experimental immunology

techniques, details of immunology measurements and appropriate instrumentation

Requirements:

Knowledge of chemistry on the level of bachelor of chemistry and completed course of fundamentals of nuclear chemistry.

Syllabus of lectures:

1.History of immunology and immunochemistry.

2.Tissues, cells and molecules of immune system

3.Regulation of immune system and its dysfunctions. Immunology deficiencies,

hyper responsiveness, immunology tolerance, autoimmunity.

4.Non-specific effector mechanisms, phagocytosis, complement system. Defence

systems based on lipids and saccharides identification.

5.Genesis and development of immunoglobulin superfamily. Immunoglobulins as

adhesive molecules.

6.Tissue antigens, HLA typization

7.T-cell receptor, membrane and soluble immunoglobulins. Immunoglobulin genes.

8.Methods of preparation and purification of antibodies. Polyclonal, monoclonal,

diabodies, single chain antibodies, minibodies. Imunologic reagents based on

platforms other than immunoglobulin domain. Characterization of interactions

antigen - antibody.

9.Types of immunoanalytical determinations.

10.Development of immunoanalytical method. Treatment and interpretation of

results.

11.Instrumentation in immunoanalytical laboratory, work organization, overview

of diagnostic application.

Syllabus of tutorials:

Study Objective:

The course provides the students with the knowledge of the fundamentals of immunochemistry and molecular imunology including the most important molecules of immunity system and technical aspects of experimental immunology techniques.

The students will acquire competence to get oriented and communicate on the interface between nuclear chemistry and immunology.

Study materials:

Key literature:

1.Paul W. E: Fundamental Immunology, 1987, Raven Press, NY, ISBN 08-28073-557-3

Recommended literature:

1.Klein J., Hořejší V.: Immunology, 2nd Ed., 1997, Blackwell, ISBN 0-632-04228-1

2.Janeway C.A., Travers P.: Immunobiology, 1994, Garlan Publishing, ISBN 0-8153-

1691-7

Note:

Time-table for winter semester 2011/2012:

Time-table is not available yet

Time-table for summer semester 2011/2012:

Time-table is not available yet

The course is a part of the following study plans: