Clinical Laboratory Instrumentation

Lecturer:

Tutor:

Supervisor:

Department of Biomedical Technology

Synopsis:

Chemistry and physical chemistry: Optical absorption. Spectrometry. Chromatography, electrophoresis, isotachophoresis. Mass spectroscopy, nuclear magnetic resonance, atomic absorption spectrometry. Electrochemistry, ion electrodes a sensors. Osmometry.

Biochemistry, hematology, immunology: Automatic chemical analyzers, blood particles counter and flow cytometry, automatic analyzers for immunoanalysis and the others techniques based on principle of detection reactions antigen, anti-matter, computer stand by analysis of findings of microscopic examination, fluorometers, luminometers, equipments for monitoring fail of blood clotting time. Acid-basic analyzers.

Biotechnology: Pressure vessels , biohazard boxes, water bath and cryostats, frigorific and freezing technology, deepfreezing boxes, lyophilisation systems, centrifuges a ultracentrifuges, laminar boxes, conductometers, pH meters, ultrapure water, inkubaton and CO2 inkubation apparatus, fermenters.

Molecular biology: Termocyclers, Real-Time PCR, DNA sequencer, analyzer of DNA/genotyper, electrophoresis, documentation systems, hybridization ovens, blotters.

Requirements:

Terms of credit: Attendance at laboratory training. Present properly elaborated protocols from laboratory training.

Terms of exam: Pass an credit. Exam includes test and verbal part.

Syllabus of lectures:

1.Principles of chemical and physical analytical methods. (Optical methods)

2.Principles of chemical and physical analytical methods. (Mass spectroscopy, Nuclear magnetic resonance. Polarography.)

3.Principles of biochemical, hematological and immunological laboratory methods (biochemical methods)

4.Principles of biochemical, hematological and immunological laboratory methods (hematological methods)

5.Methods of storage and cell cultivation in vitro, laboratory operations in v aseptic condition in biotechnological laboratories.

6.Methods of cells isolation for laboratory analysis in conditions of biotechnological laboratories.

7.Methods of molecular biology. Isolation of nucleic acids molecules and their analyses.

Syllabus of tutorials:

1.Principles of chemical and physical analytical methods. (Optical methods)

2.Principles of chemical and physical analytical methods. (Mass spectroscopy, Nuclear magnetic resonance. Polarography.)

3.Biochemical and immunological laboratory methods

4.Hematological laboratory methods

5.Methods of storage and cell cultivation in vitro, laboratory operations in v aseptic condition in biotechnological laboratories.

6.Methods of cells isolation for laboratory analysis in conditions of biotechnological laboratories.

7.Methods of molecular biology. Isolation of nucleic acids molecules and their analyses.

Study Objective:

Mastering of theoretical and practical knowledge and skills chemical and physical laboratory analytical technique, biochemical, hematological and immunological laboratory techniques. To get knowledge and practical skills in operations with laboratory equipment of biotechnological and molecular-biological laboratories. To take up with principles of bioanalytical methods in practice. Mastering of preparation and process of samples for analysis.

Study materials:

[1] Tietz Textbook of Clinical Chemistry, eds.: C.A. Burtis, E.R. Ashwood, W.B. Saunders Company, 1994, Philadelphia, London, ISBN 0 7216 4472 4

[2] Chromatography today, C.F. Poole, S.K. Poole, Elsevier, Amsterdam, 1991

[3] J.Hollas, Modern Spectroscopy, 2nd Ed., J Wiley, Chichester 1992

[4] R. Kellner, J.-M. Mermet, M. Otto, M. Valcárel, H. M. Widmer: Analytical Chemistry, 2nd edition, Wiley-VCH, Weinheim 2004

[5] BECKETT, G. - WALKER, S. W. - RAE, P., Lecture Notes - Clinical Biochemistry ISBN: 140512959X, Marston Book Services Ltd 2005

[6] Janeway, C. A. - Travers, T. - Walport, M., Immunobiology 6th Edition, ISBN: 0443073104, Elsevier Books 2004

[7] ALBERTS, B., Molecular Biology of Cell, ISBN: 0815340729,Taylor & Francis Books Ltd 2002

[8] Watson, J. - Baker, T. - Bell, S. - Gann, A., Molecular Biology of the Gene, ISBN: 080534635X, Pearson Education Ltd 2003

[9] DEVLIN, Textbook of Biochemistry with Clinical Correlation, ISBN: 0471678082, John Wiley and Sons Ltd 2005

Note:

Further information:

No time-table has been prepared for this course

The course is a part of the following study plans:

• Prospectus - magisterský (!)
• Navazující magisterský studijní obor Biomedicínský inženýr v AJ (compulsory course)