Fluorescence spectroscopy in biology and medicine

Lecturer:

Tutor:

Supervisor:

Department of Natural Sciences

Synopsis:

The course introduces students to molecular spectroscopy from its basic physical principles, main experimental approaches and working principles of common instrumentation to typical biomedical applications illustrated on selected studies published in literature. The term molecular spectroscopy is understood to refer to a a broad range of techniques investigating structure and dynamics of molecules using their interaction with electromagnetic radiation from radio-frequency to soft x-ray radiation.

Requirements:

Multiple choice test. In case of an ambiguous result followed by a question to discuss.

Syllabus of lectures:

1.Interaction of electromagnetic radiation with molecules from the point of view of classical physics, elastic scattering of light

2.Quantum-mechanical description of molecules, electronic, vibrational and rotational energy levels

3.Transitions of molecules between energy levels, selection rules

4.Absorption of electromagnetic radiation, Lambert-Beer law

5.Inelastic (Raman) scattering

6.Instrumentation for optical spectroscopy, monochromators, sources and detectors of light

7.Symmetry of molecules and selection rules of vibrational transitions, Raman scattering and IR absorption

8.Electronic transitions in visible and UV region, luminescence

9.Interpretation of optical spectra of (bio)molecules

10.Non-linear and chiroptic methods in optical spectroscopy

11.Basic principles of nuclear magnetic resonance (NMR)

12.NMR spectroscopy, one- and two-dimensional NMR spectra

13.Interpretation of NMR spectra of (bio)molecules

14.Test

Syllabus of tutorials:

1.Elemental dipole, Rayleigh scattering

2.Born-Oppenheimer approximation

3.Solution of basic quantum-mechanical problems

4.Lambert-Beer law

5.Inelastic scattering of light

6.Diffraction optics and spectrum of light

7.Molecular symmetry

8.Electronic structure of molecules

9.Interpretation of optical spectra- vibrational transitions

10.Interpretation of optical spectra - electronic transitions

11.Spin in external magnetic field

12.Introduction to interpretation of NMR spectra

13.Interpretation of NMR spectra

Study Objective:

1) Understanding basic physical principles of molecular spectroscopy and relationship between structure of a molecule and its spectra.

2) Understanding principles of main techniques of molecular spectroscopy including the working principles of typical instrumentation.

3) Introduction to typical applications of molecular spectroscopy in biomedical research and interpretation of spectroscopic data.

Study materials:

[1]Gauglitz G., Vo-Dinh T.: Handbook of Spectroscopy. Wiley VCH Verlag, Weinheim, 2003

[2]Bernath P.F., Molecular Spectroscopy and Structure, University of Waterloo (http://bernath.uwaterloo.ca/media/molspec_combined.pdf)

[3]Prosser V. et al. Experimentální metody biofyziky, Academia 1989

[4]Lecture notes available at course webpage containing links to other freely accessible web sources

Note:

Further information:

No time-table has been prepared for this course

The course is a part of the following study plans:

• Navazující magisterský studijní obor Přístroje a metody pro biomedicínu (compulsory elective course)