Bio-analytical chemistry and fundamentals of laboratory medicine

Lecturer:

Tutor:

Supervisor:

Department of Natural Sciences

Synopsis:

Subject is focused on analysis of biological material, access to analysis of biomolecules and customization of samples, methods of analysis and results evaluation in clinical laboratory.

Requirements:

Credit: 80% attendance, laboratory exercises development, successful management of credit knowledge test

Exam: oral examination

Syllabus of lectures:

1. Specifics of analysis of biological materials. Groups of biological active substances and their chemical atributes.

2. Classical and modern accesses to analysis of biomolecules. Types of bioanalytical problems. Methods for preparation of samples to bioanalysis.

3. Character of bioafinitive reactions.

Bioanalytical reagents.

4. Enzymes and enzymatic analysis. Enzymes as an analytes and analytic reagents.

Immunochemical reactions. Immunoanalysis. Recent immunoanalytic technologies.

5. Radioisotope methods. Bioafinitive sensors. Bioselective surfaces.

6. Electrochemical biosensors. Analysis of xenobiotics, fundamental of pharmacokinetic.

7.-9. Methods of separation in analysis of biological active substances. Analysis of saccharides, lipids and steroids. Analysis of nucleotides and nucleid acids.

10. Diagnostics in laboratory of molecular genetics.

11. Clinical laboratory medicine. Analytes in medicine and possibilities in laboratory dg.

12. Influences of preanalytic phase on result of laboratoty test, analytical atributes of laboratory method. Atributes of lab. method in clinical perspective.

13. Referential values of laboratory examinations.

14. Controle of qualitity of laboratory test.

Syllabus of tutorials:

1)Taking of samples and treatment of biological material in laboratory maedicine

2) Pre-analytic phase of laboratory examination

3) Biochemical and morphological examination of urine

4) Examination of acidobasic balance

5) Determination of ions and inorganic substances

6) Laboratory assesment of enzymes I

7) Laboratory assesment of enzymes II

8) Immunochemistry methods

9) The toxicological examination

10) Determination of drugs and TDM

11) Examination of haemocoagulation

12) The basic haematological examination, haematological analysers

13) Immunohaematological diagnostics

14) Credit knowledge test

Study Objective:

Study goal is introduction with diferent types of materials evaluated in clinical laboratory, teoretical introduction with methods used in clinical laboratory practice and their evaluation and their practical aplication some of them

Study materials:

Basice literature

RACEK, J. A KOL.: Klinická biochemie. Galén. Praha 2006.

SCHNEIDERKA, P. A KOL.: Stanovení analytů v medicíně. Karolinum, Praha 2002.

PRŮŠA, R. A KOL.: Průvodce laboratorními nálezy. RAABE, Praha 2010.

ZIMA, T. A KOL.: Laboratorní diagnostika. Galén Karolinum, Praha 2007

DOLEŽALOVÁ A KOL.: Laboratorní technika v klinické biochemii a toxikologii, IDVPZ Brno, Brno 1995.

PECKA, M.: Přehled laboratorní hematologie I-IV. Galén, Praha 2005.

BARTŮŇKOVÁ, J.: Vyšetřovací metody v imunologii. Grada Publishing, Praha 2005.

Recomended literature:

JABOR, A. A KOL.: Vnitřní prostředí. Grada, Praha 2008

ŠTERN, P. A KOL: Obecná a klinická biochemie pro bakalářské obory studia. Karolinum, Praha 2005.

MASOPUST, J.: Klinická biochemie - požadování a hodnocení biochemických vyšetření I+II.díl. Karolinum, Praha 1998.

VODRÁŽKA, Z.: Biochemie. Academia, Praha 1992. MURRAY R.K. ET AL.: Harperova biochemie, české vydání. H+H, Praha 2002. KODÍČEK, M.: Biochemické pojmy - výkladový slovník. Praha, Vydavatelství VŠCHT, 2004.

KUKAČKA J., KLAPKOVÁ E., KOTAŠKA K.: Bioanalytická chemie v příkladech a cvičeních. Karolinum, Praha, 2010 ISBN 978-80-246-1853-1 DOLEŽALOVÁ A KOL.: Principy biochemických vyšetřovacích metod, IDVPZ Brno, Brno 1995.

Note:

Further information:

No time-table has been prepared for this course

The course is a part of the following study plans:

• Navazující magisterský studijní obor Přístroje a metody pro biomedicínu (compulsory course)