Advanced Spectroscopy and Microscopy for Molecular medicine

Lecturer:

Tutor:

Supervisor:

Department of Natural Sciences

Synopsis:

The course provides an overview of the most representative advanced optical spectroscopy methods with a high time resolution (ns, ps, fs) along with the techniques that combine such a time-resolution with a high spatial resolution. Also single-molecule and single-particle spectroscopy are covered as well as selected nonlinear spectroscopy methods - basic 2D techniques and 3-pulse fs photo echo. In particular, physical interpretation of such spectroscopic data is discussed together with a character of information on bio-functional molecular systems that can be acquired by applying an advanced optical spectroscopy.

The course is suitable especially for PhD students who are members of basic-research teams active in the core fields of molecular medicine such as chemistry, molecular photophysics and biophysics, physiology, pharmacology, etc.

Requirements:

Syllabus of lectures:

I.Molecular photophysics and the type of information that can be acquired by optical spectroscopy: overview.

II.Basic techniques to achieve the high resolution (spectral, time, and spatial) in optical spectroscopy methods.

III.Selected topics I: High (ns, ps, fs) time-resolution methods for biologically important molecular systems

IV.Selected topics II: Methods with a high spatial resolution and single-molecule spectroscopy.

V.Molecular-level study of the key biological processes in real time.

Syllabus of tutorials:

Study Objective:

Study materials:

Fleming G. R., Kaufman L. J.: Ultrafast Spectroscopy. Oxford University Press, 2005 a 2009

Hof M., Hutterer R., Fidler V: Fluorescence Spectroscopy in Biology. Springer Verlag,

Heidelberg, 2004

Partioe navazující na vybrané příklady z:

Corkum P. at all. (Eds.): Ultrafast Phenomena XV /2007/ a XVI /2009/, Springer Verlag

Note:

Further information:

No time-table has been prepared for this course

The course is a part of the following study plans:

• Prospectus - doktorský (!)