Microbiology and imunology

Lecturer:

Veronika Vymětalová (gar.)

Tutor:

Veronika Vymětalová (gar.), Markéta Zezulová

Supervisor:

Department of Natural Sciences

Synopsis:

Microbiology: Organization and function in microbial cell. Procaryotic cell, binary fission. Phylogenetic system of bacteria and archea. Cyanobacteria. Microbial nutrition, microbial growth - life cycle of procaryotic cell, growth curve, kinetics of bacterial growth. Influence of environmental factors on microbial growth - temperature, pH, extreme environment. Antimicrobial, antibacterial agents - antibiotics and mechanisms of their effect, dezinfection and sterilization. Energetic metabolism. Genetics and molecular biology of microorganisms. Microflora of human body. Microbial diseases and their control.

Immunology: Cells and organs of the immune system. Antigens. Immune reaction. Main histocompatibility complex. B-lymphocytes. Production of immunoglobulins. Immunoglobulins, function and structure. T-lymphocytes and cellular immunity. Cytotoxic lymphocytes. Cytokines. Immunity against infection. Non-specific immunity. Allergic diseases. Immunity and nutrition. Immunopathology. Immunodeficiency. Autoimmune diseases.

Requirements:

Assessment: laboratory practices elaboration, succesful mastery of written work.

Examination: class ticket, succesful mastery of written work

Syllabus of lectures:

1.Microbiology: Organization and function in microbial cell. Procaryotic cell, binary fission. Phylogenetic system of bacteria and archea. Cyanobacteria.

2.Microbial nutrition, microbial growth - life cycle of procaryotic cell, growth curve, kinetics of bacterial growth.

3.Influence of environmental factors on microbial growth - temperature, pH, extreme environment. Antimicrobial, antibacterial agents - antibiotics and mechanisms of their effect, dezinfection and sterilization.

4.Energetic metabolism. Genetics and molecular biology of microorganisms.

5.Microflora of human body.

6.Microbial diseases and their control.

7.Immunology: Cells and organs of the immune system.

8.Antigens.

9.Immune reaction.

10.Main histocompatibility complex. B-lymphocytes. Production of immunoglobulins. Immunoglobulins, function and structure. T-lymphocytes and cellular immunity. Cytotoxic lymphocytes. Cytokines.

11.Immunity against infection. Non-specific immunity. Allergic diseases. Immunity and nutrition.

12.Immunopathology. Immunodeficiency. Autoimmune diseases.

Syllabus of tutorials:

1. Sterilization and desinfection methods.

2. Basic praxis in microbiological laboratory.

3. Bacterial DNA isolation.

4. Bacterial cultivation.

5. Gram staining.

6. Imonogenetics - excercices

Study Objective:

Micro-organism, microflora of human body, microbial disease

Study materials:

Rosypal S. a kol.: Obecná bakteriologie. SPN Praha, 1981.

Šilhánková L: Mikrobiologie pro potravináře a biotechnology. Victoria Publishing Praha, 1995.

Votava M. a Ondrovčík P.: Vybrané kapitoly z klinické mikrobiologie. MU Brno, 1998.

Němec M. a Horáková D.: Základy mikrobiologie. MU Brno, 1999.

Kaprálek F. : Základy bakteriologie, UK Praha , 2000.

Votava M. Lékařská mikrobiologie obecná, Neptun 2001, Brno

Ferenčík M. a kol.: Imunitní systém. Praha. Grada. 2005

Hořejší V., Bartůňková J: Základy imunologie. 3. vyd. Triton, Praha 2005

Note:

Time-table for winter semester 2011/2012:

Time-table is not available yet

Time-table for summer semester 2011/2012:

	06:00–08:0008:00–10:0010:00–12:0012:00–14:0014:00–16:0016:00–18:0018:00–20:0020:00–22:0022:00–24:00
Mon
Tue	roomKL:B-507 Vymětalová V. 14:00–15:50 (lecture parallel1) Kladno FBMI Učebna
Fri
Thu	roomKL:B-104 Vymětalová V. Zezulová M. 09:00–10:50 (lecture parallel1 parallel nr.1) Kladno FBMI Mikroskopická praktikaroomKL:B-104 Vymětalová V. Zezulová M. 11:00–12:50 (lecture parallel1 parallel nr.2) Kladno FBMI Mikroskopická praktika
Fri

The course is a part of the following study plans:

• Bakalářský studijní obor Optika a optometrie - prezenční (compulsory course)