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CZECH TECHNICAL UNIVERSITY IN PRAGUE
STUDY PLANS
2019/2020

Principles of Microscopic Methods

The course is not on the list Without time-table
Code Completion Credits Range Language
17PBLPMM Z 2 2P Czech
Lecturer:
Tutor:
Supervisor:
Department of Natural Sciences
Synopsis:

Students will learn various types of microscopic methods used in biological sciences and medicine. Light microscopy techniques, techniques of electron microscopy (SEM and TEM) in biology, fluorescence microscopy, confocal microscopy and more.

Requirements:

Credit requirements: 2 knowledge tests during the semester, drawing up written answers to questions, with a success rate of 60%.

Syllabus of lectures:

1st Light microscopy techniques

2nd Principle of other types of microscopy (phase contrast, darkfield)

3rd The physical principle of the microscope

4th Preparations for light microscopy.

5th Inverted microscope and its application in biology and medicine

6th Fluorescence microscopy.

7th Scanning probe microscopy, AFM, confocal microscopy

8th The techniques of electron microscopy - TEM.

9th The techniques of electron microscopy - SEM

10th Microscopic immunodetection in Biomedicine

Syllabus of tutorials:

Subject does not exercise.

Study Objective:

The aim of the course is to provide students with a comprehensive interpretation of microscopic methods used in biological sciences and medicine. Understand the principles of the techniques of light microscopy, fluorescence microscopy, confocal microscopy and electron microscopy (SEM, TEM) used in biology and medicine.

Study materials:

Povinná literatura:

1.NEBESÁŘOVÁ, Jana. Elektronová mikroskopie pro biology [online]. České Budějovice: 13.1.2002/13.1.2002. http://www.paru.cas.cz/lem/book/

2.Hejtmánek, M.: Úvod do světelné mikroskopie. Skripta UP Olomouc, 2001.

Doporučená literatura:

1.Amelinckx,S., D. van Dyck, J. van Landuyt, G. van Tendeloo. Handbook of Microscopy: Applications in Materials Science, Solid-State Physics and Chemistry Applications.VCH Verlagsgesellschaft mbH, 1997. Ebook - http://knihovny.cvut.cz - EIZ - Wiley online library.

2.Molecular Expressions - Optical Microscopy Primer - Introduction [online]. 19.6.1998/16.1.2009. http://micro.magnet.fsu.edu/primer/index.html

3.KAK, A.C., SLANEY, M. Principles of Computerized Tomographic Imaging [online]. http://www.slaney.org/pct/pct-toc.html

Note:
Further information:
No time-table has been prepared for this course
The course is a part of the following study plans:
Data valid to 2020-01-23
For updated information see http://bilakniha.cvut.cz/en/predmet2744706.html