- Enrollement in the course requires an successful completion of the following courses:
- General Chemistry and Basic Laboratory Calculations (17PBLOZLA)
- Department of Biomedical Technology
Course participants will be introduced to the basics of Biochemistry. The course builds on the knowledge gained in general chemistry and extends this knowledge about the chemistry of living systems. The interpretation goes through the basic building structures of biological systems (amino acids, peptides, proteins, lipids, carbohydrates, nucleic acids), biological membranes and molecular genetics to the most important metabolic processes. Particular attention is paid to the aspects necessary for understanding the methods of work in the biochemical and clinical laboratory, which are part of the follow-up chemical discipline.
The laboratories are focused on broadening the topics discussed in the lectures and their practical training, especially on the determination of biomolecules and the verification of their properties. Students should become familiar with the basic laboratory techniques of Biochemistry.
Obligatory and active attendance at all tutorials. Absences are acceptable for serious reasons only and must be apologized and recompensed.
At the beggining of each tutorial, student has to pass an entrance test at least for 50 %. In the other way, he has to recompense the tutorial and the test on an other date. Student can recompense maximally two tutorials.
Student has to write and submit protocols from all lab sessions in sufficient quality.
The exam consists of a written test and covers the topics of all lectures and tutorials.
Qualification of the test corresponds to the valid ECTS grading scale.
Detail information in „Demands for passing the subject“ document.
- Syllabus of lectures:
1. Introduction to the biochemistry.
2. Aminoacids, peptides and proteins I
3. Aminoacids, peptides and proteins II
5. Nuceic acids, basics of molecular genetics
6. Lipids and biomembranes
8. Basic principles of matebolism, bioenergetic
9. Respiratory chain, Krebs cycle
10. Metabolism of nitrogen compound. Test from proteins and aminoacids. Basic separation and analytical methods of proteins
- Syllabus of tutorials:
1st Properties and reactions of biologically important organic compounds
2nd Color reactions of amino acids
3rd Reversible and irreversible protein denaturation
4th zolace proteins from natural materials
6th Isolation of lipid fractions from a natural material
7th Properties lipids
8th The chemical properties of carbohydrates
9th Enzyme kinetics
10th Isolation of DNA and RNA from a natural material
- Study Objective:
The goal of study is to understand the significance of biochemical compounds in living organisms, and to deduce their properties by the knowledge of their structure. After passing the course, student should be well versed in biochemical problems either of clinical diagnostics either of daily life. Further aim of this subject is to get work with scientific publications, search for biochemical information and to present obtained knowledge to other students.
- Study materials:
1.Štern, P., et al: General and Clinical Biochemistry courses for undergraduate studies. University Press, Cambridge, 2005.
2.Racek, J. et al. Clinical Biochemistry. Galen. Prague 2006
3.Ledvina, M., Stoklasová, A., Cerman, J. biochemistry for students of medicine. Karolinum. Prague 2008.
1.Kodíček, M. Biochemical terms - explanatory dictionary. Prague Institute of Chemical Technology Publishing House, 2004.
2.Jabor, A. et al. Indoor environment. Grada. Prague 2008
- Further information:
- No time-table has been prepared for this course
- The course is a part of the following study plans: