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CZECH TECHNICAL UNIVERSITY IN PRAGUE
STUDY PLANS
2018/2019

Clinical Laboratory Instrumentation

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Code Completion Credits Range Language
17PBBLT Z,ZK 4 2+2 Czech
Grading of the course requires grading of the following courses:
Biochemistry (17PBBBCH)
Physical Chemistry (17PBBFCH)
Lecturer:
Lenka Strnadová (guarantor)
Tutor:
Lenka Strnadová (guarantor), Zuzana Fílová
Supervisor:
Department of Biomedical Technology
Synopsis:

Clinical laboratory instrumentation introduces principles of bioanalytical methods used in clinical diagnostics. Emphasis is put on optical methods (UV-VIS spectrophotometry, IR spectroscopy, AAS, AES, fluorimetry), NMR and X-ray analysis, electrochemical and electromigration methods (ion electrodes, biosensors, electrophoresis, isoelectric focusing), imunoassays and genetic methods (ELISA, PCR) as well as on chromatography and mass spectrometry. Contribution of lab automation to clinical diagnostics will be also discussed.

During the laboratory course students will be introduced into the basics of work in bioanalytical laboratory and lab data processing.

Requirements:

Credit requirements:

Active attendance at all tutorials. Students has to come in time and be well prepared. Absences are acceptable for serious reasons only, have to be apologized and recompensed. Passing entrance test at least for 50% for each tutorial; if not, the tutorial including the test will be recompensed on another date.

Working out assigned exercises and submiting laboratory reports in satisfying quality.

Examination conditions: Student has to pass a written test comprising the topics discussed during the lectures and tutorials, including assigned individual study. The exam is graded according to the ECTS scale, to pass the exam, the test must be evaluated at least at 50 %.

Syllabus of lectures:

1. Princles of bioanalytical methods and clinical diagnostic

2. Optical methods I

3. Optical methods II

4. Electrochemical methods (galvanic sond for dissolved oxygen measurement, alternatives for Clark probe, advantages and disadvantages of its use, methods of calibration)

5. Electrochemical methods for measuring blood gases (Astrup, instrumentation of methodd, used electrodes - its type and construction, calculation of potentials of electrodes, gas CO2 probe - specifics of measurement, factors affectig measurement, right experimantal conditions setup)

6. Biosensors and automation

7. Electromigration methods

8. Genetic techniques

9. Imunochemical methods

10. Membrane processes

11. Centrifugation and extraction

12. Chromatography I

13. Chromatography II

14. Massí spectrometry

Syllabus of tutorials:

1. Principles of bioanalytical methods in a clinical laboratory

2. UV-VIS spectrometry, fluorimetry, AAS and AES, turbidimetry and nephelometry

3. Electromigration techniques

4. Electrochemical techniques

5. Electrochemical techniques of measurement dissolved gases (Measurement of concentration/partial pressure of CO2 with gas probe, Measurement of partial pressure of O2 with galvanic probe)

6. Genetic techniques

7. Chromatography

Study Objective:

Goal of the study is to get to know the principles of common bioanalytical methods used in a clinical laboratory, to work with scientific text and to discuss the current topics, and mainly, to solve model laboratory problems and tasks, which may happen in clinical diagnostics.

Study materials:

1. Kenkel, John. Analytical chemistry for technicians. 4th ed. Boca Raton: CRC Press/Taylor & Francis, 2014. ISBN 978-1-4398-8105-7

2. Mikkelsen, S. R., Cortón, E.: Bioanalytical Chemistry. Wiley & Sons, Inc. Hoboken 2004.

3. Arneson, W., Brickell, J.: Clinical Chemistry - A Laboratory Perspective. F. A. Davis Company, Philadelphia 2007.

4. Gauglitz, G., Vo-Dinh, T.: Handbook of Spectroscopy. Wiley & Sons, Inc. Hoboken 2003.

5. Günzler, H., Williams, A.: Handbook of Analytical Techniques. Wiley & Sons, Inc. Hoboken 2001.

6. Nelson, D. L., Cox, M. M.: Lehninger principles of biochemistry. W. H. Freeman, New York 2008.

7. Voet, D., Voet, J. G.: Biochemistry. Wiley & Sons, Inc. Hoboken 2004.

Note:
Time-table for winter semester 2018/2019:
Time-table is not available yet
Time-table for summer semester 2018/2019:
06:00–08:0008:00–10:0010:00–12:0012:00–14:0014:00–16:0016:00–18:0018:00–20:0020:00–22:0022:00–24:00
Mon
roomKL:A-7
Fílová Z.
14:00–17:50
EVEN WEEK

(lecture parallel1
parallel nr.4)

Kladno FBMI
Laboratoř chemických předmětů
Tue
roomKL:A-7
Fílová Z.
10:00–13:50
EVEN WEEK

(lecture parallel1
parallel nr.1)

Kladno FBMI
Laboratoř chemických předmětů
roomKL:A-7
Fílová Z.
10:00–13:50
ODD WEEK

(lecture parallel1
parallel nr.5)

Kladno FBMI
Laboratoř chemických předmětů
Fri
roomKL:A-007
Fílová Z.
08:00–11:50
EVEN WEEK

(lecture parallel1
parallel nr.6)

Kladno FBMI
Laboratoř chemických předmětů
roomKL:A-7
Fílová Z.
08:00–11:50
ODD WEEK

(lecture parallel1
parallel nr.7)

Kladno FBMI
Laboratoř chemických předmětů
Thu
roomKL:C-4
Strnadová L.
12:00–13:50
(lecture parallel1)
Kladno FBMI
Malý sál
Fri
The course is a part of the following study plans:
Data valid to 2019-06-18
For updated information see http://bilakniha.cvut.cz/en/predmet2169106.html